| Abstract (English): |
The experiment aims to investigate the effect of monoglyceride monolaurate (ML) on gene expression of 3D4/21 macrophages infected with porcine epidemic diarrhea virus (PEDV). After determining the optimal concentration and optimal time by ML on 3D4/21 cell viability experimental and PEDV growth curve in 3D4/21 cells, 3D4/21 cells were randomly divided into three groups (control group, PEDV group, PEDV+ML group), and each group was treated with both full-time addition of 10 µmol/L ML and 10 µmol/L ML after PEDV-infected cells, respectively. And the expression of related genes were detected. The results showed that the addition of 10 µmol/L ML significantly promoted the growth of 3D4/21 cells (P<0.05), and the relative expression of PEDV-S, M and N genes showed a tendency of increasing and then decreasing during 48 h of virus infection of 3D4/21 cells, and reached the highest at 12 h. Compared with the PEDV group, the relative expressions of PEDV-S, M, N, IL-8, IFN-β, and IFITM3 genes were significantly down-regulated in the full-time addition of ML treatment for 12 h in the PEDV+ML group (P< 0.05); the relative expressions of PEDV-S, M, N, IL-8, IFN-β, MX1, ISG15, IFIT1, IFITM1 genes were significantly down-regulated (P<0.05) and the relative expression of KCNJ13 gene was significantly upregulated after treatment for 24 h (P<0.05). Compared with the PEDV group, the relative expression of MMP13 gene was significantly down-regulated (P<0.05), and the relative expression of PEDV-M gene was significantly up-regulated in the PEDV+ML group by adding ML for 12 h after infection (P<0.05); the relative expressions of IL-6, IL-8, IFN- β, MX1, ISG15, IFIT1, IFITM1 gene were significantly down-regulated (P<0.05) and the relative expressions of PEDV-S, M, N, KCNJ13 genes were significantly up-regulated after treatment for 24 h (P<0.05). In summary, ML pretreatment before PEDV infection has a certain antiviral effect; PEDV infection puts cells in an immune stress state, and ML has a certain alleviating effect. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
试验旨在研究单月桂酸甘油酯 (ML) 对猪流行性腹泻病毒 (PEDV) 感染3D4/21巨噬细胞 基因表达的影响。通过ML对3D4/21细胞活力试验和PEDV在3D4/21细胞中的生长曲线确定最适浓 度和最佳时间后，将 3D4/21细胞随机分为 3组 (control组、PEDV 组、PEDV+ML 组), 各组分别以全时 添加10 µmol/L ML和PEDV感染细胞后添加10 µmol/L ML两种方式进行处理，并检测相关基因相对 表达量。结果表明：添加10 µmol/L的ML对3D4/21细胞生长具有显著的促进作用 (P<0.05), 病毒感染 3D4/21细胞的48 h内 PEDV-S、M、N基因相对表达量呈现先增加再降低的趋势，12 h时处于最高；与 PEDV组相比，PEDV+ML组全时添加ML处理12 h使PEDV-S、M、N、IL-8、IFN-β、IFITM3基因相对表 达量显著下调 (P<0.05), 处理24 h使PEDV-S、M、N、IL-8、IFN-β、MX1、ISG15、IFIT1和IFITM1基因相对 表达量显著下调 (P<0.05), KCNJ13基因相对表达量显著上调 (P<0.05) 。与PEDV组相比，PEDV+ML组 在感染后添加ML处理12 h使MMP13基因相对表达量显著下调 (P<0.05), PEDV-M基因相对表达量显 著上调 (P<0.05), 处理24 h使IL-6、IL-8、IFN-β、MX1、ISG15、IFIT1、IFITM1、IFITM3基因相对表达量显 著下调 (P<0.05), PEDV-S、M、N、KCNJ13基因相对表达量显著上调 (P<0.05) 。综上所述，PEDV感染前 ML预处理具有一定的抗病毒效果；PEDV感染使细胞处于免疫应激状态，ML有一定的缓解作用。 [ABSTRACT FROM AUTHOR] |