التفاصيل البيبلوغرافية
| العنوان: |
Full-length target sequences of GeoMx digital spatial profiling probes reveal that gene-promiscuity predicts probe sensitivity to EDTA tissue decalcification. |
| المؤلفون: |
Oszwald, André1 (AUTHOR), Zisser, Lucia2 (AUTHOR), Schachner, Helga1 (AUTHOR), Kaltenecker, Christopher1 (AUTHOR), Wasinger, Gabriel1 (AUTHOR), Rohrbeck, Johannes1 (AUTHOR), Kozakowsky, Nicolas1 (AUTHOR), Tiefenbacher, Andreas1 (AUTHOR), Rees, Andrew J.1 (AUTHOR), Kain, Renate1 (AUTHOR) renate.kain@meduniwien.ac.at |
| المصدر: |
Scientific Reports. 9/10/2024, Vol. 14 Issue 1, p1-8. 8p. |
| مصطلحات موضوعية: |
*TRANSCRIPTOMES, *GENE targeting, *TISSUES, *HUMAN beings |
| مستخلص: |
GeoMx Digital Spatial Profiling (Nanostring) is a commercial spatial transcriptomics method to selectively analyze regions of interest within intact tissue sections. We show that decalcification with ethylene-diamine-tetra-acetic (EDTA) variably attenuates probe counts, while probes that are more resistant to this effect consequently appear overexpressed after quantile normalization. By determining the undisclosed full-length target sequences of probes used in the human whole transcriptome panel, hereby updating target transcripts and genes, we find that the gene-promiscuity of probes is an important factor that determines sensitivity to EDTA incubation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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