| Abstract (English): |
[Objectives]This article aimed to obtain characteristics and high-quality germplasm resources of Flammulina filiformis by space mutagenesis technology. [Methods]F.filiformis strain F3-1 was subjected to aerospace mutagenesis. Spaceflight mutagenesis strains were screened, characterized and analyzed for diversity of genetic variations by experiments such as antagonistic experiments, molecular marker experiments and determination of active substances. Strains with high contents of active substances were screened. [Results]Seven variants with obvious antagonism to the starting strain were obtained by antagonism test. Molecular marker(ISSR, RAPD, SRAP)clustering analysis showed that the seven mutant strains and starting strain shared genetic similarity coefficients from 0.694 to 0.962, and could be classified into three clusters at a genetic similarity coefficient of 0.800. The variation coefficients such as mycelial growth rate, biomass, phenolics, flavanoid, polysaccharide and ergothionein between mutagenized and starting strains ranged from 7.68% to 38.97%, and the genetic diversity index H' ranged from 1.53 to 2.00. Among them, the highest coefficient of variation was found for polysaccharide content, indicating that it was greatly affected by mutagenesis; the genetic diversity index was largest for total phenols, indicating its rich mutagenesis types. Biological properties cluster analysis revealed three clusters at a genetic distance of 3.16. Finally, two high polysaccharide content strains(T2 and T5)were obtained by screening, among which T2 was superior to the starting strain in terms of growth rate, biomass and active substances, while T5 was mainly characterized as a high polysaccharide content strain with 114.70% higher polysaccharide content than the starting strain(P<0.01). [Conclusions]There was abundant genetic variation diversity in the strains treated by spaceflight mutagenesis, which was an excellent germplasm resource for new variety breeding. [ABSTRACT FROM AUTHOR] |