التفاصيل البيبلوغرافية
| العنوان: |
干旱胁迫对芋球茎淀粉和蔗糖 代谢通路相关基因表达的影响. |
| Alternate Title: |
Effects of drought stress on expression of genes related to starch and sucrose metabolic pathways in taro corms. |
| المؤلفون: |
董伟清1 dwq5899@126.com, 刘莉莉2, 邱祖杨2, 何芳练1 hefanglian0480@163.com |
| المصدر: |
Southwest China Journal of Agricultural Sciences. 2024, Vol. 37 Issue 4, p738-747. 10p. |
| Abstract (English): |
[Objective] The study aimed to investigate the molecular mechanism of taro corm in response to drought stress, especially the effect of drought on the expression of genes related to starch and sucrose metabolic pathways, in order to provide a basis for the selection and breeding of drought-tolerant varieties. [Method) Using Lipo taro 1 as test material, transcriptome sequencing was performed on der drought stress, and the differentially expressed genes obtained were analyzed by GO and KEGG enrichment, to further genes related to starch and sucrose metabolism pathways, and to analyze the expression patterns of these enzyme genes under drought stress, [Result] Taro screened for 1983 differentially expressed genes under drought stress, including 373 up-regulated and 1610 down-regulated genes. GO enrichment analysis showed that differentially expressed genes mast enriched in biological processes for cellular processes and metabolic processes, in cellular components for cellular anatomical entity, and in molecular functions for catalytic activity and binding, KEGG enrichment analysis showed that the top enriched pathways were metabolic pathways, nitrogen metabolism, ascorbate and al darate metabolism. The differentially expressed genes of starch and sucrose metabolic pathways counted, and a total of 26 differentially expressed genes involving 11 enzymes screened, of which 5 up-regulated expressed genes and 21 were down-regulated expressed genes. Under drought stress, genes for starch synthase, hexokinase, fructokinase, UTP-glucose-l-phosphate uridylyltransferase, α-amylase, en doglucanase, β-glucosidase and β-fructofuranosidase w expressed in a down-regulated manner; Genes for UDP-glucose glucophosphohydro-lase and trehalose-6-phosphate synthase/phosphatase up-regulated; And genes for sucrose synthase and β-furanofuranosidase pressed in both up-regulated and down-regulated manners. [Conclusion] Expression of genes related to starch and sucrose metabolic pathways was affected by drought stress. Genes involved in the regulation of starch metabolism and alginate biosynthesis were down-regulated, whereas genes involved in the regulation of sucrose metabolism were both up and down-regulated. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
【目的】採完半球茎响应干旱胁迫的分子机制,特别是干旱胁迫对淀粉和產糖代谢通路相关基因表达的影响,为耐旱品种 选育提供依据。【方法】以荔浦芋1号为试验材料,对干旱胁迫下的平球茎进行转录组测序,对获得的差异表达基因进行60和 KEGG 富集分析,进一步筛选与淀粉和蔗糖代谢通路相关的酶基因,并分析这些酶基因在千早静地下的表达模式。【结果】平球茎 在干旱胁迫下筛选到差异表达基因1983个,其中上调表选基因373个,下调表达基因1610个。GO富集分析显示,差异表达基因 在生物过程富基最多的是细胞过程和代谢过程,在细胞组分富集最多的是细胞解到实体,在分子功能富集最多的催化活性和结 合。KEGG 富集分析显示,富集最多的通路分別是代谢通路、氮素代谢通路、抗坏血酸和醛酸代谢通路。对淀粉和蔗糖代谢通路的 差异表达基因进行统计,共筛选到26个差异表达基因,涉及11种陶,其中上调表达基因5个,下调表达基因21个。在干旱胁迫 下,淀粉合成酶、已糖激酶、果糖激酶、UTP-4-磷酸盐尿苷酸转移酶、淀粉酶、内切葡聚糖酶、B-葡萄糖苷酶和β-呋喃果糖 苷酶的基因下调表达;UDP-葡萄糖磷酸水解酶和海藻-6-磷酸合成/磷酸酶的基因上调表达;蔗糖合成酶和B-呋喃果糖苷酶的基因 既有上调表达,也有下调表达。【结论】淀粉和蔗糖代谢通路相关基因的表达受到手早胁迫的影响。调控淀粉代谢和海產糖生物 合成相关基因下调表达,而调控采糖代谢相关基因既有上调表达,也有下调表达。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |