التفاصيل البيبلوغرافية
| العنوان: |
鸢尾素对心肌梗死后大鼠心房颤动和心房间质纤维化的影响. |
| Alternate Title: |
Effects of Irisin on Atrial Fibrillation and Interstitial Fibrosis in Rats after Myocardial Infarction. |
| المؤلفون: |
任 何1 renheH0326@126.com, 程 锦1, 张明明1, 张东伟1, 张 薇1, 耿倩雯2 gengqwen@163.com |
| المصدر: |
Progress in Modern Biomedicine. 2022, Vol. 22 Issue 22, p4229-4234. 6p. |
| مصطلحات موضوعية: |
*CARDIAC hypertrophy, *STAINS & staining (Microscopy), *MYOCARDIAL infarction, *ATRIAL fibrillation, *WESTERN immunoblotting |
| Abstract (English): |
Objective: To explore the effects of irisin on atrial fibrillation and interstitial fibrosis in rats after myocardial infarction. Methods: A rat model of acute myocardial infarction (MI) was established by ligation of the left anterior descending coronary artery (LADCA). Rats were intraperitoneally injected with irisin at 100 μg/kg daily for 4 weeks. Left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular ejection fraction (LVEF), and left ventricular fractional shortening (LVFS) were examined by echocardiography. The rat myocardial morphology and fibrosis were evaluated by hematoxylin-eosin (HE) staining and Masson's trichrome staining. Western blot analysis was used to detect the expression of TGF-β1, Smad2, p-Smad2, Smad3, p-Smad3, MMP9, TIMP-1, collagen Ⅰ, collagen Ⅲ, NLRP3, Caspase 1, and IL-1β. Results: Compared with the model group, the LVEF and LVFS of the irisin group were significantly increased, while the LVEDD and LVESD were significantly decreased (P<0.05). The induction rate and duration of atrial fibrillation in the irisin group were significantly reduced (P<0.05). The myocardial morphology of the irisin group was significantly improved, and the fibrosis area was significantly reduced (P<0.05). The protein expression levels of TGFβ1, p-Smad2/3, collagen Ⅰ, collagen Ⅲ and MMP9 in the irisin group were significantly reduced, while the protein expression level of TIMP1 was significantly increased (P<0.05). Moreover, the expression levels of NLRP3 inflammasome, cleaved-caspase-1, and IL-1β in irisin group were significantly reduced (P<0.05). Conclusion: Irisin could significantly improve the cardiac function, inhibit the myocardial fibrosis and hypertrophy, and inhibit the formation of atrial fibrillation in an animal model of myocardial infarction. Thus, it has a high clinical application prospect. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的: 探讨鸢尾素对心肌梗死后大鼠心房颤动和心房间质纤维化的影响。方法: 通过结扎左前降支冠状动脉 (LADCA) 建立急性心肌梗死 (MI)大鼠模型, 大鼠每天腹腔注射 100μg/kg 的鸢尾素1次, 共治疗4周。通过超声心动图检查左室舒张末期直径 (LVEDD)、左室收缩末期直径 (LVESD)、左室射血分数 (LVEF)、左室缩短分数 (LVFS)。应用苏木精-伊红 (HE) 染色和Masson三色染色评估大鼠心肌形态和纤维化情况。蛋白质印迹分析检测 TGF-β1、Smad2、p-Smad2、Smad3、p-Smad3、MMP9、TIMP-1、collagenⅠ、collagenⅢ、NLRP3、Caspase 1 和 IL-1β 的表达。结果: 与模型组相比, 鸢尾素组大鼠的 LVEF 和 LVFS 升高, 而 LVEDD 和 LVESD 降低 (P＜0.05); 房颤诱发率和房颤持续时间显著降低 (P＜0.05); 心肌形态明显改善, 纤维化面积显著降低 (P＜0.05); TGFβ1、p-Smad2/3、collagenⅠ、collagenⅢ 和 MMP9 的蛋白表达水平显著降低, TIMP1 的蛋白表达水平显著升高 (P＜0.05); NLRP3 炎性体、cleaved-caspase-1 和 IL-1β 的表达水平显著降低 (P＜0.05)。结论: 鸢尾素可显著改善心肌梗死动物模型的心脏功能, 抑制心肌纤维化和房颤形成, 具有较高的临床应用前景。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |