التفاصيل البيبلوغرافية
| العنوان: |
Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation. |
| المؤلفون: |
Chen, Genghua1,2 (AUTHOR), Yin, Yunqian1,2 (AUTHOR), Lin, Zetong1,2 (AUTHOR), Wen, Huaqiang1,2 (AUTHOR), Chen, Jiahui1,2 (AUTHOR), Luo, Wen1,2 (AUTHOR) luowen729@scau.edu.cn |
| المصدر: |
Biochemical & Biophysical Research Communications. Jun2021, Vol. 559, p84-91. 8p. |
| مصطلحات موضوعية: |
*MYOBLASTS, *MUSCLE growth, *TRANSCRIPTION factors, *CELL cycle, *SKELETAL muscle, *PROTEIN expression |
| مستخلص: |
Skeletal muscle development is a sophisticated multistep process orchestrated by diverse myogenic transcription factors. Recent studies have suggested that Kelch-like genes play vital roles in muscle disease and myogenesis. However, it is still unclear how Kelch-like genes impact myoblast physiology. Here, through integrative analysis of the mRNA expression profile during chicken primary myoblast and C2C12 differentiation, many differentially expressed genes were found and suggested to be enriched in myoblast differentiation and muscle development. Interestingly, a little-known Kelch-like gene KLHL30 was screened as skeletal muscle-specific gene with essential roles in myogenic differentiation. Transcriptomic data and quantitative PCR analysis indicated that the expression of KLHL30 is upregulated under myoblast differentiation state. KLHL30 overexpression upregulated the protein expression of myogenic transcription factors (MYOD , MYOG , MEF2C) and induced myoblast differentiation and myotube formation, while knockdown of KLHL30 caused the opposite effect. Furthermore, KLHL30 was found to significantly decrease the numbers of cells in the S stage and thereby depress myoblast proliferation. Collectively, this study highlights that KLHL30 as a muscle-specific regulator plays essential roles in myoblast proliferation and differentiation. • KLHL30, KLHL31 and KLHL40 are induced during myogenic differentiation. • KLHL30 decreased myoblast proliferation and cell cycle progression. • KLHL30 increased expression of MYOD, MYOG, MEF2C and MyHC in myoblasts. • KLHL30 promoted myoblast fusion and myogenic differentiation. • This study highlights the essential role of KLHL30 on myoblast differentiation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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