التفاصيل البيبلوغرافية
| العنوان: |
AKAP-Lbc Nucleates a Protein Kinase D Activation Scaffold |
| المؤلفون: |
Carnegie, Graeme K., Smith, F. Donelson smithdon@ohsu.edu, McConnachie, George1, Langeberg, Lorene K.1, Scott, John D.1 |
| المصدر: |
Molecular Cell. Sep2004, Vol. 15 Issue 6, p889-899. 11p. |
| مصطلحات موضوعية: |
*PROTEIN kinases, *PHOSPHOTRANSFERASES, *IMMUNOGLOBULINS, *PHOSPHORYLATION |
| مستخلص: |
The transmission of cellular signals often proceeds through multiprotein complexes where enzymes are positioned in proximity to their upstream activators and downstream substrates. In this report we demonstrate that the A-kinase anchoring protein AKAP-Lbc assembles an activation complex for the lipid-dependent enzyme protein kinase D (PKD). Using a combination of biochemical, enzymatic, and immunofluorescence techniques, we show that the anchoring protein contributes to PKD activation in two ways: it recruits an upstream kinase PKCη and coordinates PKA phosphorylation events that release activated protein kinase D. Thus, AKAP-Lbc synchronizes PKA and PKC activities in a manner that leads to the activation of a third kinase. This configuration illustrates the utility of kinase anchoring as a mechanism to constrain the action of broad-spectrum enzymes. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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