التفاصيل البيبلوغرافية
| العنوان: |
FgHtf1 regulates global gene expression towards aerial mycelium and conidiophore formation in the cereal fungal pathogen Fusarium graminearum. |
| المؤلفون: |
Gaili Fan1,2, Huawei Zheng3, Kai Zhang4,5, Ganeshan, Veena Devi6, Opiyo, Stephen Obol7, Liu, Dan1, Mingyang Li3, Guangpu Li8, Mitchell, Thomas K.6, Yingzi Yun1, Zonghua Wang1,3 wangzh@fafu.edu.cn, Guo-dong Lu1 |
| المصدر: |
Applied & Environmental Microbiology. May2020, Vol. 86 Issue 9, p1-35. 47p. |
| مصطلحات موضوعية: |
*GENE expression, *HOMEOBOX genes, *CHITIN synthase, *FUSARIUM, *GENE families, *MYCELIUM |
| مستخلص: |
The homeobox gene family of transcription factors (HTF) control many developmental pathways and physiological processes in eukaryotes. We previously showed that a conserved HTF (FgHtf1) regulates conidia morphology in the plant pathogenic Fusarium graminearum. This study investigates the mechanism of FgHtf1-mediated regulation and identifies putative FgHtf1 target genes by chromatin immunoprecipitation assay combined with parallel DNA sequencing (ChIP-seq) and RNA sequencing. A total of 186 potential binding peaks, including 142 genes directly regulated by FgHtf1 were identified. Subsequent motif prediction analysis identified two DNA binding motifs TAAT and CTTGT. Among the FgHtf1 target genes included FgHTF1 itself and several important conidiation related genes g. FgCON7), the chitin synthase pathway genes, and the aurofusarin biosynthetic pathway genes. In addition, FgHtf1 may regulate the cAMP-PKA-Msn2/4 and Ca2+40 -calcineurin-Crz1 pathways. Taken together, these results suggest that in addition to auto-regulation, FgHtf1 also controls global gene expression, and promotes a shift to aerial growth and conidiation in graminearum by activation of FgCON7 or other conidiation related genes. [ABSTRACT FROM AUTHOR] |
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