Academic Journal

Analysis of cytotoxic effects of nickel on human blood lymphocytes.

التفاصيل البيبلوغرافية
العنوان: Analysis of cytotoxic effects of nickel on human blood lymphocytes.
المؤلفون: Zarei, Mohammad Hadi1 (AUTHOR), Hosseini Shirazi, Seyed Farshad1 (AUTHOR), Aghvami, Marjan1 (AUTHOR), Salimi, Ahmad2 (AUTHOR) salimikd@yahoo.com, Pourahmad, Jalal1 (AUTHOR) j.pourahmadjaktaji@utoronto.ca
المصدر: Toxicology Mechanisms & Methods. Feb2018, Vol. 28 Issue 2, p79-86. 8p. 3 Charts, 5 Graphs.
مصطلحات موضوعية: *NICKEL in the body, *INDUSTRIAL toxicology, *FOOD toxicology, *NICKEL compounds, *LYMPHOCYTES, *METAL toxicology, *PROTEOLYSIS
مستخلص: Nickel compounds possess many applications in different industrial processes. Human beings are exposed to nickel commonly through occupational exposure and food. Although a few studies so far have investigated the effects of nickel compounds on human lymphocytes, the complete mechanism of cytotoxicity of this metal on human lymphocytes is yet to be determined. The intention of this paper was to determine the cytotoxicity mechanism of water soluble NiCl2 toward human lymphocytes using the accelerated cytotoxicity mechanisms screening (ACMS) technique. Human lymphocytes were isolated from the blood of healthy subjects based on Ficoll-Paque PLUS standard method. For the assessment of cell viability, lymphocytes were incubated with 0.05–1 mM NiCl2 for 12 h. Determination of mechanistic parameters was performed 2, 4 and 6 h after treatment of cells with ½ EC5012h, EC5012h and 2EC5012h of NiCl2. Our results demonstrate that cytotoxicity of NiCl2 on human lymphocytes is associated with increased ROS formation, mitochondrial membrane potential collapse, glutathione depletion, lysosomal membrane damage, cellular proteolysis and activation of caspase-3 before cytotoxicity ensued. [ABSTRACT FROM AUTHOR]
قاعدة البيانات: Academic Search Index
الوصف
تدمد:15376516
DOI:10.1080/15376516.2017.1364314