التفاصيل البيبلوغرافية
| العنوان: |
Development of a self-assembling protein nanoparticle vaccine targeting Plasmodium falciparum Circumsporozoite Protein delivered in three Army Liposome Formulation adjuvants. |
| المؤلفون: |
Seth, Labdhi1, Bingham Ferlez, Karen M.1, Kaba, Stephen A.1, Musser, Derek M.2, Emadi, Sharareh3, Matyas, Gary R.4, Beck, Zoltan5, Alving, Carl R.4, Burkhard, Peter6, Lanar, David E.7 david.e.lanar.civ@mail.mil |
| المصدر: |
Vaccine. Oct2017, Vol. 35 Issue 41, p5448-5454. 7p. |
| مصطلحات موضوعية: |
*DRUG development, *PROTOZOAN vaccines, *TARGETED drug delivery, *NANOMEDICINE, *LIPOSOMES, *PLASMODIUM falciparum, *CIRCUMSPOROZOITE protein, *MOLECULAR self-assembly |
| مستخلص: |
We have developed FMP014, a vaccine candidate against Plasmodium falciparum malaria, which is comprised of 60 identical monomer protein chains that form an icosahedral shaped self-assembling protein nanoparticle (SAPN). Each monomer contains selected P. falciparum Circumsporozoite Protein (P f CSP) CD4+ and CD8+ epitopes, universal T H epitopes, portions of the α-TSR domain, and 6 repeats of the NANP motifs of the P f CSP. Here we describe the conditions that are required for successful scale-up and cGMP manufacturing of FMP014 with a yield of ≈1.5 g of drug substance per 100 g of wet bacterial paste. When adjuvanted with an Army Liposomal Formulation (ALF) based adjuvant, the nanoparticle vaccine is highly immunogenic and prevents infection of mice by an otherwise lethal dose of transgenic P. berghei sporozoites expressing the full-length P f CSP. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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