التفاصيل البيبلوغرافية
| العنوان: |
Fengycin produced by Bacillus subtilis 9407 plays a major role in the biocontrol of apple ring rot disease. |
| المؤلفون: |
Fan, Haiyan1 fanhaiyan6860@163.com, Ru, Jinjiang1 rjj19871017@126.com, Zhang, Yuanyuan1 465422523@qq.com, Wang, Qi1 wangqi@cau.edu.cn, Li, Yan1 liyancau@gmail.com |
| المصدر: |
Microbiological Research. Jun2017, Vol. 199, p89-97. 9p. |
| مصطلحات موضوعية: |
*APPLE disease & pest prevention, *BIOLOGICAL pest control agents, *FENGYCIN, *BACILLUS subtilis, *TRANSPOSONS, *BACTERIA |
| مستخلص: |
Apple ring rot, caused by Botryosphaeria dothidea , is a serious apple disease in China. Bacillus subtilis 9407 was isolated from healthy apples and showed strong antifungal activity against B. dothidea . To identify the primary antifungal compound of B. subtilis 9407 and determine its role in controlling apple ring rot, a transposon mutant library was constructed using Tn YLB-1 , and a mutant completely defective in antifungal activity was obtained. The gene inactivated in the antifungal activity mutant had 98.5% similarity to ppsB in B. subtilis subsp. subtilis str. 168, which encodes one of the five synthetases responsible for synthesizing fengycin. A markerless ppsB deletion mutant was constructed. Compared with the wild-type strain, lipopeptide crude extracts from Δ ppsB showed almost no inhibition of B. dothidea mycelial growth. Furthermore, fengycin-like lipopeptides (retention factor 0.1–0.2) that exhibited antifungal activity against B. dothidea were observed in the wild-type strain by thin-layer chromatography (TLC)-bioautography analysis, but not in Δ ppsB . Semipreparative reverse-phase high performance liquid chromatography (RP-HPLC) detection revealed that Δ ppsB lost the ability to synthesize fengycin. These results suggest that ppsB is responsible for synthesizing fengycin and that fengycin is the major antifungal compound produced by B. subtilis 9407 against B. dothidea. Moreover, a biocontrol assay showed that the control efficacy of Δ ppsB was reduced by half compared with the wild-type strain, indicating that fengycin plays a major role in controlling apple ring rot disease. This is the first report on the use of a B. subtilis strain as a potential biological control agent to control apple ring rot disease by the production of fengycin. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder