التفاصيل البيبلوغرافية
| العنوان: |
上调 miR-34c-5p 对鼻咽癌细胞 HONE-1 增殖和侵袭的影响. |
| Alternate Title: |
The effect of up-regulating miR-34c-5p on the proliferation and invasion of nasopharyngeal carcinoma cell line HONE-1. |
| المؤلفون: |
马菲菲1, 郝彦强2, 王国强3, 张鑫4, 郅程5, 冀天星6 |
| المصدر: |
Tianjin Medical Journal. Oct2015, Vol. 43 Issue 10, p1097-1099. 4p. |
| Abstract (English): |
Objective To investigate the effect of up-regulating miR-34c-5p on cloning formation and migration of nasopharyngeal carcinoma cell line HONE-1 in vitro. Methods Expression levels of miR-34c-5p in nasopharyngeal cell line NP69 and nasopharyngeal carcinoma cell line HONE-1 were investigated by real time quantitative PCR;Cloning Formation Test and Transwell Migration Assay were used to explore the effect of up-regulating miR-34c-5p on proliferation and migration of nasopharyngeal carcinoma cell line HONE-1. Results The expression of miR-34c-5p in nasopharyngeal carcinoma cell line HONE-1 was lower than that in normal nasopharyngeal cell line NP69. Up-regulating miR-34c-5p significantly suppressed the cloning formation and migration capacity, compared to those of NP69 cell line and HONE-1 with normal level of miR-34c-5p(P<0.05). Conclusion Down-regulating miR-34c-5p involve in proliferation and migration of nasopharyngeal carcinoma and may be a used as a new therapeutic target for nasopharyngeal carcinoma. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的：探讨上调miR-34c-5p对鼻咽癌细胞HONE-1增殖和转移能力的影响。方法利用定量PCR分析miR-34c-5p在鼻咽癌细胞株HONE-1和正常鼻咽细胞株NP69中的表达情况；利用克隆形成实验和Transwell实验分析上调miR-34c-5p表达对miR-34c-5p mimic转染组、miR-34c-5p对照序列组和未处理组增殖和侵袭能力的影响。结果 miR-34c-5p在鼻咽癌细胞株HONE-1的表达水平低于正常鼻咽细胞株NP69；miR-34c-5p mimic转染组鼻咽癌细胞HONE-1的克隆形成和侵袭能力明显低于miR-34c-5p对照序列组和未处理组（均P＜0.05）。结论 miR-34c-5p的下调与鼻咽癌的生物学功能增殖和侵袭能力相关，是鼻咽癌治疗的潜在靶点。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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