التفاصيل البيبلوغرافية
| العنوان: |
Consideration of Diet Was Necessary for the Successful Isolation and Culture of Weddell Seal (Leptonychotes weddellii) Myoblasts. |
| المؤلفون: |
Garcia, Teresa M.1, Green, Todd L.1, De Miranda Jr., Michael A.1, Pearson, Linnea E.2, Cuevas, Lauren M.1, Larson, Ashley M.1, Kanatous, Shane B.1 shane.kanatous@colostate.edu |
| المصدر: |
Aquatic Mammals. 2014, Vol. 40 Issue 4, p412-422. 11p. |
| مصطلحات موضوعية: |
*MYOBLASTS, *WEDDELL seal, *CELL culture, *CELL proliferation, *PHYSIOLOGY, *DIET |
| مستخلص: |
We developed an isolation and culture method for Weddell seal (Leptonychotes weddellii) myoblasts and discovered that isolated Weddell seal cells proliferate poorly after initial plating and freezing without lipid supplementation in media, which is reflective of their high-fat diets. We tested the effects of media lipid concentrations ranging from 2.5 to 10% on the number of myotubes formed between days 1 and 7 of differentiation. We determined that the growth and differentiation media for cultured Weddell seal skeletal muscle cells must be supplemented with a lipid concentration between 2.5 and 5%. This modification to standard growth and differentiation media formulations illustrates the importance of considering the diet of non-model organisms in primary culture. The success of these techniques provides a unique opportunity to investigate molecular regulatory pathways essential to physiological responses seen in the whole animal. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |