التفاصيل البيبلوغرافية
| العنوان: |
Enzyme-linked immunospot assay for the diagnosis of active Treponema pallidum infection during the various stages of syphilis. |
| المؤلفون: |
Tabidze IL (AUTHOR), Lee FK (AUTHOR), Tambe P (AUTHOR), Rocha E (AUTHOR), Larsen SA (AUTHOR), Stoll BJ (AUTHOR), St Louis ME (AUTHOR), Nahmias AJ (AUTHOR), Tabidze, I L1 (AUTHOR), Lee, F K (AUTHOR), Tambe, P (AUTHOR), Rocha, E (AUTHOR), Larsen, S A (AUTHOR), Stoll, B J (AUTHOR), St Louis, M E (AUTHOR), Nahmias, A J (AUTHOR) |
| المصدر: |
Sexually Transmitted Diseases. Sep99, Vol. 26 Issue 8, p426-430. 5p. |
| مستخلص: |
Background: Specific serologic assays for syphilis cannot differentiate current infections from past infections and are inefficient to monitor efficacy of antibiotic therapy.Goal: To develop a new immunologic assay for the identification of active Treponema pallidum infection during the various stages of syphilis.Study Design: Peripheral blood mononuclear cells obtained from patients with syphilis in an STD clinic were tested for T. pallidum-specific circulating antibody-secreting cells (ASC) by an enzyme-linked immunospot assay (ELISPOT).Results: Specific ASC were demonstrated in all six patients with primary syphilis and in 14 of 16 patients diagnosed with secondary syphilis. ASCs were undetectable in five patients 8 to 16 days after appropriate therapy, but persisted in one case that was considered treatment failure. Among the 13 patients diagnosed with latent syphilis, six (46%) demonstrated ASC, reflecting antigenic stimulation.Conclusion: The ELISPOT assay is effective for the diagnosis of primary and secondary syphilis. The presence of circulating ASC suggests persistent active infection in some patients during the latent disease stage. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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