التفاصيل البيبلوغرافية
| العنوان: |
An Affinity Reagent for the Recognition of Pyrophosphorylated Peptides. |
| المؤلفون: |
Conway, John H.1, Fiedler, Dorothea1 dfiedler@princeton.edu |
| المصدر: |
Angewandte Chemie International Edition. Mar2015, Vol. 54 Issue 13, p3941-3945. 5p. |
| مصطلحات موضوعية: |
*PYROPHOSPHORYLASES, *PEPTIDES, *ENCAPSULATION (Catalysis), *CHEMICAL reagents, *CARBOXYLIC acids |
| مستخلص: |
A resin-bound dinuclear zinc(II) complex for the selective capture of pyrophosphopeptides is reported. The metal complex binds diphosphate esters over other anionic groups, such as monophosphate esters, sulfate esters, and carboxylic acids, with high specificity. Immobilization of the compound provided a reagent capable of binding and retaining nanomolar quantities of pyrophosphopeptide in the presence of cell lysate. The high affinity and specificity of the reagent makes it an attractive tool for the study of in vivo pyrophosphorylation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |