التفاصيل البيبلوغرافية
| العنوان: |
Preventive effects of CTLA4Ig-overexpressing adipose tissue--derived mesenchymal stromal cells in rheumatoid arthritis. |
| المؤلفون: |
EUN WHA CHOI1,2 vet.cew@gmail.com, TAE WON YUN1, JI WOO SONG1, MINJAE LEE1, JEHOON YANG1,2, KYU-SIL CHOI1,2 |
| المصدر: |
Cytotherapy (Elsevier Inc.). Mar2015, Vol. 17 Issue 3, p271-282. 12p. |
| مصطلحات موضوعية: |
*RHEUMATOID arthritis, *GENE expression, *MESENCHYMAL stem cells, *CELLULAR therapy, *KERATINOCYTES, *LABORATORY mice, *PREVENTION |
| مستخلص: |
Background aims. Rheumatoid arthritis is a systemic autoimmune disorder. In this study, we first compared the therapeutic effects of syngeneic and xenogeneic adipose tissue--derived stem cells on a collagen-induced arthritis mouse model. Second, we investigated the synergistic preventive effects of CTLA4Ig and adipose tissue--derived mesenchymal stromal cells (ASCs) as a therapeutic substance. Methods. Arthritis was induced in all groups except for the normal, saline (N) group, using chicken type II collagen (CII). Animals were divided into C (control, saline), H (hASCs), M (mASCs) and N groups (experiment I) and C, H, CT (CTLA4Ig-overexpressing human ASC [CTLA4Ig-hASCs]) and N groups (experiment II), according to transplanted material. Approximately 2 x 106 ASCs or 150 |xL of saline was intravenously administered on days 24, 27, 30 and 34, and all animals were killed on days 42 to 44 after CII immunization. Results. Anti-mouse CII autoantibodies were significantly lower in the H, M and CT groups than in the C group. Cartilage damage severity score and C-telopeptide of type II collagen were significantly lower in the CT group than in the C group. The serum levels of IL-6 were significantly lower in the H, M and CT groups than in the C group. The serum levels of keratinocyte chemoattractant were significantly lower in the CT group than the C group. Conclusions. There were similar effects of ASCs on the decrease of anti-mouse CII autoantibody levels between syngeneic and xenogeneic transplantations, and CTLA4Ig-hASCs showed synergistic preventive effects compared with non-transduced hASCs. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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