Academic Journal
Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance in clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae
| العنوان: | Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance in clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae |
|---|---|
| المؤلفون: | Yuhan, Yin, Ziyun, Yue, Yongbo, Zhang, Fuqiang, Li, Qinghua, Zhang |
| المصدر: | Revista da Sociedade Brasileira de Medicina Tropical. April 2016 49(2) |
| بيانات النشر: | Sociedade Brasileira de Medicina Tropical - SBMT, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Tigecycline, Resistance mechanism, Efflux pump, Acinetobacter baumannii, Klebsiella pneumonia |
| الوصف: | INTRODUCTION: Due to the wide use of tigecycline in the treatment of severe infections caused by multidrug-resistant (MDR) bacteria, clinical resistance to tigecycline has increased in recent years. Here, we investigated the relationship between tigecycline resistance and the expression of efflux pumps. METHODS: Clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae were consecutively collected from hospitalized patients in three hospitals. The minimum inhibitory concentration (MIC) of tigecycline was determined using the broth microdilution method. Expression levels of efflux pump genes and regulators were examined by quantitative real-time reverse transcription polymerase chain reaction. The correlations between tigecycline MICs and gene expression levels were analyzed. RESULTS: Overall, 1,026 A. baumannii and 725 K. pneumoniae strains were collected. Most strains were isolated from sputum. The tigecycline resistance rate was 13.4% in A. baumannii isolates and 6.5% in K. pneumoniae isolates. Overexpression of AdeABC and AcrAB-TolC efflux systems was observed found in clinical tigecycline-resistant isolates. The tigecycline MIC had a linear relationship with the adeB expression level in A. baumannii isolates, but not with the acrB expression level in K. pneumoniae isolates. There were significant linear trends in the overexpression of ramA as the tigecycline MIC increased in K. pneumoniae isolates. CONCLUSIONS: Tigecycline resistance in A. baumannii and K. pneumoniae was strongly associated with the overexpression of efflux systems. More studies are needed to elucidate whether there are other regulators that affect the expression of adeB in A. baumannii and how ramA affects the expression of acrB in K. pneumoniae. |
| نوع الوثيقة: | article |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 0037-8682 |
| DOI: | 10.1590/0037-8682-0411-2015 |
| URL الوصول: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000200165 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edssci.S0037.86822016000200165 |
| قاعدة البيانات: | SciELO |
| ResultId |
1 |
|---|---|
| Header |
edssci SciELO edssci.S0037.86822016000200165 900 3 Academic Journal academicJournal 899.816589355469 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edssci&AN=edssci.S0037.86822016000200165&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000200165# [Name] => EDS - SciELO [Category] => fullText [Text] => View record in SciELO [MouseOverText] => View record in SciELO ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance in clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Yuhan%2C+Yin%22">Yuhan, Yin</searchLink><br /><searchLink fieldCode="AR" term="%22Ziyun%2C+Yue%22">Ziyun, Yue</searchLink><br /><searchLink fieldCode="AR" term="%22Yongbo%2C+Zhang%22">Yongbo, Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Fuqiang%2C+Li%22">Fuqiang, Li</searchLink><br /><searchLink fieldCode="AR" term="%22Qinghua%2C+Zhang%22">Qinghua, Zhang</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <i>Revista da Sociedade Brasileira de Medicina Tropical</i>. April 2016 49(2) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Sociedade Brasileira de Medicina Tropical - SBMT, 2016. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2016 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Tigecycline%22">Tigecycline</searchLink><br /><searchLink fieldCode="DE" term="%22Resistance+mechanism%22">Resistance mechanism</searchLink><br /><searchLink fieldCode="DE" term="%22Efflux+pump%22">Efflux pump</searchLink><br /><searchLink fieldCode="DE" term="%22Acinetobacter+baumannii%22">Acinetobacter baumannii</searchLink><br /><searchLink fieldCode="DE" term="%22Klebsiella+pneumonia%22">Klebsiella pneumonia</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => INTRODUCTION: Due to the wide use of tigecycline in the treatment of severe infections caused by multidrug-resistant (MDR) bacteria, clinical resistance to tigecycline has increased in recent years. Here, we investigated the relationship between tigecycline resistance and the expression of efflux pumps. METHODS: Clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae were consecutively collected from hospitalized patients in three hospitals. The minimum inhibitory concentration (MIC) of tigecycline was determined using the broth microdilution method. Expression levels of efflux pump genes and regulators were examined by quantitative real-time reverse transcription polymerase chain reaction. The correlations between tigecycline MICs and gene expression levels were analyzed. RESULTS: Overall, 1,026 A. baumannii and 725 K. pneumoniae strains were collected. Most strains were isolated from sputum. The tigecycline resistance rate was 13.4% in A. baumannii isolates and 6.5% in K. pneumoniae isolates. Overexpression of AdeABC and AcrAB-TolC efflux systems was observed found in clinical tigecycline-resistant isolates. The tigecycline MIC had a linear relationship with the adeB expression level in A. baumannii isolates, but not with the acrB expression level in K. pneumoniae isolates. There were significant linear trends in the overexpression of ramA as the tigecycline MIC increased in K. pneumoniae isolates. CONCLUSIONS: Tigecycline resistance in A. baumannii and K. pneumoniae was strongly associated with the overexpression of efflux systems. More studies are needed to elucidate whether there are other regulators that affect the expression of adeB in A. baumannii and how ramA affects the expression of acrB in K. pneumoniae. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => text/html ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0037-8682 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1590/0037-8682-0411-2015 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000200165" linkWindow="_blank">http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000200165</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => info:eu-repo/semantics/openAccess ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edssci.S0037.86822016000200165 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1590/0037-8682-0411-2015
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Tigecycline
[Type] => general
)
[1] => Array
(
[SubjectFull] => Resistance mechanism
[Type] => general
)
[2] => Array
(
[SubjectFull] => Efflux pump
[Type] => general
)
[3] => Array
(
[SubjectFull] => Acinetobacter baumannii
[Type] => general
)
[4] => Array
(
[SubjectFull] => Klebsiella pneumonia
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance in clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yuhan, Yin
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ziyun, Yue
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yongbo, Zhang
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Fuqiang, Li
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Qinghua, Zhang
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 04
[Type] => published
[Y] => 2016
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 00378682
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 49
)
[1] => Array
(
[Type] => issue
[Value] => 2
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Revista da Sociedade Brasileira de Medicina Tropical
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |