Patent
Peptide reagent for the detection of human cytomegalovirus (CMV)
| العنوان: | Peptide reagent for the detection of human cytomegalovirus (CMV) |
|---|---|
| Patent Number: | 6,936,251 |
| تاريخ النشر: | August 30, 2005 |
| Appl. No: | 10/288220 |
| Application Filed: | November 05, 2002 |
| مستخلص: | The invention relates to a peptide reagent comprising peptides immunochemically reactive with antibodies to the human cytomegalovirus (CMV). New antibodies directed to said peptides or fragments thereof are also part of the invention. Also cell lines capable of producing monoclonal antibodies are part of the invention. The invention also relates to a method for the detection of CMV or antibodies directed against CMV in a test fluid and a test kit to be used when applying the said detection methods. Detection of CMV in a test fluid or tissue specimen using antibodies, monoclonal and polyclonal, directed to said peptide, which have the characteristics of detecting both native and denatured DMV proteins are also part of said invention. |
| Inventors: | Middeldorp, Jaap Michiel (Oss, NL); Van De Crommert, Johannes Martinus Gerardus (Veghel, NL) |
| Assignees: | bioMerieux, B.V. (Boxtal, NL) |
| Claim: | 1. An isolated peptide reagent, comprising a first peptide comprising the amino acid sequence of SEQ ID NO:1, a second peptide comprising the amino acid sequence of SEQ ID NO:7 and a third peptide comprising the amino acid sequence of SEQ ID NO:10. |
| Claim: | 2. A peptide reagent, consisting of a first peptide consisting of the amino acid sequence of SEQ ID NO:1, a second, separate peptide consisting of the amino acid sequence of SEQ ID NO:7, and a third, separate peptide consisting of the amino acid sequence of SEQ ID NO:10. |
| Claim: | 3. A method for the detection of antibodies to CMV in a sample, comprising contacting the peptide reagent of claim 1 with the sample under conditions whereby an antigen/antibody complex can form and detecting formation of an antigen/antibody complex, thereby detecting antibodies to CMV in the sample. |
| Claim: | 4. A method for the detection of antibodies to CMV in a sample, comprising contacting the peptide reagent of claim 2 with the sample under conditions whereby an antigen/antibody complex can form and detecting formation of an antigen/antibody complex, thereby detecting antibodies to CMV in the sample. |
| Claim: | 5. A test kit comprising the peptide reagent of claim 1 . |
| Claim: | 6. A test kit comprising the peptide reagent of claim 2 . |
| Current U.S. Class: | 4241/861 |
| Patent References Cited: | 5744298 April 1998 Stuber et al. 5859185 January 1999 Stuber et al. 6120989 September 2000 Vornhagen et al. 3619902 March 1988 4128684 April 1993 4426453 November 1995 236145 September 1987 0 248 909 December 1987 0 330 051 August 1989 WO 92/00323 January 1992 |
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| Assistant Examiner: | Lucas, Zachariah |
| Primary Examiner: | Housel, James |
| Attorney, Agent or Firm: | Myers, Bigel, Sibley & Sajovec, P.A. |
| رقم الانضمام: | edspgr.06936251 |
| قاعدة البيانات: | USPTO Patent Grants |
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