Academic Journal
A novel regulator of wheat tillering LT1 identified by using an upgraded BSA method, uni-BSA.
| العنوان: | A novel regulator of wheat tillering LT1 identified by using an upgraded BSA method, uni-BSA. |
|---|---|
| المؤلفون: | Yuan, Yundong, Lyu, Bo, Qi, Juan, Liu, Xin, Wang, Yuanzhi, Delaplace, Pierre, Du, Yanfang |
| المصدر: | Molecular Breeding, 44 (7), 47 (2024-06-04) |
| بيانات النشر: | Springer Science and Business Media B.V., 2024. |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Auxin, Bulked segregant analysis, Cytokinin, Sucrose, Tillering, Wheat, Whole-exome resequencing, Analysis method, Axillary meristem, Plant architecture, Resequencing, Biotechnology, Molecular Biology, Genetics, Agronomy and Crop Science, Plant Science, Life sciences, Agriculture & agronomy, Sciences du vivant, Agriculture & agronomie |
| الوصف: | [en] UNLABELLED: Branching/tillering is a critical process for plant architecture and grain yield. However, Branching is intricately controlled by both endogenous and environmental factors. The underlying mechanisms of tillering in wheat remain poorly understood. In this study, we identified Less Tiller 1 (LT1) as a novel regulator of wheat tillering using an enhanced bulked segregant analysis (BSA) method, uni-BSA. This method effectively reduces alignment noise caused by the high repetitive sequence content in the wheat genome. Loss-of-function of LT1 results in fewer tillers due to defects in axillary meristem initiation and bud outgrowth. We mapped LT1 to a 6 Mb region on the chromosome 2D short arm and validated a nucleotide-binding (NB) domain encoding gene as LT1 using CRISPR/Cas9. Furthermore, the lower sucrose concentration in the shoot bases of lt1 might result in inadequate bud outgrowth due to disturbances in the sucrose biosynthesis pathways. Co-expression analysis suggests that LT1 controls tillering by regulating TaROX/TaLAX1, the ortholog of the Arabidopsis tiller regulator REGULATOR OF AXILLARY MERISTEM FORMATION (ROX) or the rice axillary meristem regulator LAX PANICLE1 (LAX1). This study not only offers a novel genetic resource for cultivating optimal plant architecture but also underscores the importance of our innovative BSA method. This uni-BSA method enables the swift and precise identification of pivotal genes associated with significant agronomic traits, thereby hastening gene cloning and crop breeding processes in wheat.SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-024-01484-7. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://link.springer.com/content/pdf/10.1007/s11032-024-01484-7.pdf; urn:issn:1380-3743; urn:issn:1572-9788 |
| DOI: | 10.1007/s11032-024-01484-7 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/320345 |
| Rights: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsorb.320345 |
| قاعدة البيانات: | ORBi |
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However, Branching is intricately controlled by both endogenous and environmental factors. The underlying mechanisms of tillering in wheat remain poorly understood. In this study, we identified Less Tiller 1 (LT1) as a novel regulator of wheat tillering using an enhanced bulked segregant analysis (BSA) method, uni-BSA. This method effectively reduces alignment noise caused by the high repetitive sequence content in the wheat genome. Loss-of-function of LT1 results in fewer tillers due to defects in axillary meristem initiation and bud outgrowth. We mapped LT1 to a 6 Mb region on the chromosome 2D short arm and validated a nucleotide-binding (NB) domain encoding gene as LT1 using CRISPR/Cas9. Furthermore, the lower sucrose concentration in the shoot bases of lt1 might result in inadequate bud outgrowth due to disturbances in the sucrose biosynthesis pathways. 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