Electronic Resource
Impaired Excitatory Neurotransmission in the Urinary Bladder from the Obese Zucker Rat: Role of Cannabinoid Receptors
| العنوان: | Impaired Excitatory Neurotransmission in the Urinary Bladder from the Obese Zucker Rat: Role of Cannabinoid Receptors |
|---|---|
| المؤلفون: | Agustín Guerrero-Hernandez, Blaha, Igor, Recio Visedo, María Paz, Martínez Sainz, María Del Pilar, López-Oliva Muñoz, María Elvira, Fernandes Ribeiro, Ana Sofía, Agis Torres, Ángel, Martínez Gómez, Ana Cristina, Benedito Castellote, Sara, García Sacristán, Albino, Fernandes, Vítor S., Hernández Rodríguez, Medardo Vicente |
| بيانات النشر: | San Francisco, CA : Public Library of Science 2024-01-31T18:00:50Z 2024-01-31T18:00:50Z 2016-06-10 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Metabolic syndrome (MS) is a known risk factor for lower urinary tract symptoms. This study investigates whether functional and expression changes of cannabinoid CB1 and CB2 receptors are involved in the bladder dysfunction in an obese rat model with insulin resistance. Bladder samples from obese Zucker rat (OZR) and their respective controls lean Zucker rat (LZR) were processed for immunohistochemistry and western blot for studying the cannabinoid receptors expression. Detrusor smooth muscle (DSM) strips from LZR and OZR were also mounted in myographs for isometric force recordings. Neuronal and smooth muscle CB1 and CB2 receptor expression and the nerve fiber density was diminished in the OZR bladder. Electrical field stimulation (EFS) and acetylcholine (ACh) induced frequency- and concentration-dependent contractions of LZR and OZR DSM. ACh contractile responses were similar in LZR and OZR. EFS-elicited contractions, however, were reduced in OZR bladder. Cannabinoid receptor agonists and antagonists failed to modify the DSM basal tension in LZR and OZR In LZR bladder, EFS responses were inhibited by ACEA and SER-601, CB1 and CB2 receptor agonists, respectively, these effects being reversed by ACEA plus the CB1 antagonist, AM-251 or SER-601 plus the CB2 antagonist, AM-630. In OZR bladder, the inhibitory action of ACEA on nerve-evoked contractions was diminished, whereas that SER-601 did not change EFS responses. These results suggest that a diminished function and expression of neuronal cannabinoid CB1 and CB2 receptors, as well as a lower nerve fiber density is involved in the impaired excitatory neurotransmission of the urinary bladder from the OZR. Depto. de Enfermería Fac. de Enfermería, Fisioterapia y Podología TRUE pub |
| مصطلحات الفهرس: | 61, 612.019, Ciencias Biomédicas, Fisiología veterinaria, 24 Ciencias de la Vida, journal article, VoR |
| URL: | PR6/13-18858 Universidad Complutense de Madrid |
| الاتاحة: | Open access content. Open access content Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 open access |
| ملاحظة: | application/pdf 1932-6203 English |
| Other Numbers: | ESRCM oai:docta.ucm.es:20.500.14352/97370 Blaha I, Recio P, Martínez MP, López-Oliva ME, Ribeiro ASF, Agis-Torres Á, et al. (2016) Impaired Excitatory Neurotransmission in the Urinary Bladder from the Obese Zucker Rat: Role of Cannabinoid Receptors. PLoS ONE 11(6): e0157424. https://doi.org/10.1371/journal.pone.0157424 1932-6203 10.1371/journal.pone.0157424 1429623957 |
| المصدر المساهم: | REPOSITORIO E-PRINTS UNIVERSIDAD COMPLU From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1429623957 |
| قاعدة البيانات: | OAIster |
| ResultId |
1 |
|---|---|
| Header |
edsoai OAIster edsoai.on1429623957 948 3 Electronic Resource electronicResource 947.880004882813 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsoai&AN=edsoai.on1429623957&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://hdl.handle.net/20.500.14352/97370# [Name] => EDS - OAIster [Category] => fullText [Text] => View record in OAIster [MouseOverText] => View record in OAIster ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Impaired Excitatory Neurotransmission in the Urinary Bladder from the Obese Zucker Rat: Role of Cannabinoid Receptors
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Agustín+Guerrero-Hernandez%22">Agustín Guerrero-Hernandez</searchLink><br /><searchLink fieldCode="AR" term="%22Blaha%2C+Igor%22">Blaha, Igor</searchLink><br /><searchLink fieldCode="AR" term="%22Recio+Visedo%2C+María+Paz%22">Recio Visedo, María Paz</searchLink><br /><searchLink fieldCode="AR" term="%22Martínez+Sainz%2C+María+Del+Pilar%22">Martínez Sainz, María Del Pilar</searchLink><br /><searchLink fieldCode="AR" term="%22López-Oliva+Muñoz%2C+María+Elvira%22">López-Oliva Muñoz, María Elvira</searchLink><br /><searchLink fieldCode="AR" term="%22Fernandes+Ribeiro%2C+Ana+Sofía%22">Fernandes Ribeiro, Ana Sofía</searchLink><br /><searchLink fieldCode="AR" term="%22Agis+Torres%2C+Ángel%22">Agis Torres, Ángel</searchLink><br /><searchLink fieldCode="AR" term="%22Martínez+Gómez%2C+Ana+Cristina%22">Martínez Gómez, Ana Cristina</searchLink><br /><searchLink fieldCode="AR" term="%22Benedito+Castellote%2C+Sara%22">Benedito Castellote, Sara</searchLink><br /><searchLink fieldCode="AR" term="%22García+Sacristán%2C+Albino%22">García Sacristán, Albino</searchLink><br /><searchLink fieldCode="AR" term="%22Fernandes%2C+Vítor+S%2E%22">Fernandes, Vítor S.</searchLink><br /><searchLink fieldCode="AR" term="%22Hernández+Rodríguez%2C+Medardo+Vicente%22">Hernández Rodríguez, Medardo Vicente</searchLink> ) Array ( [Name] => PublisherInfo [Label] => Publisher Information [Group] => [Data] => San Francisco, CA : Public Library of Science 2024-01-31T18:00:50Z 2024-01-31T18:00:50Z 2016-06-10 ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => Electronic Resource ) Array ( [Name] => Abstract [Label] => Abstract [Group] => Ab [Data] => Metabolic syndrome (MS) is a known risk factor for lower urinary tract symptoms. This study investigates whether functional and expression changes of cannabinoid CB1 and CB2 receptors are involved in the bladder dysfunction in an obese rat model with insulin resistance. Bladder samples from obese Zucker rat (OZR) and their respective controls lean Zucker rat (LZR) were processed for immunohistochemistry and western blot for studying the cannabinoid receptors expression. Detrusor smooth muscle (DSM) strips from LZR and OZR were also mounted in myographs for isometric force recordings. Neuronal and smooth muscle CB1 and CB2 receptor expression and the nerve fiber density was diminished in the OZR bladder. Electrical field stimulation (EFS) and acetylcholine (ACh) induced frequency- and concentration-dependent contractions of LZR and OZR DSM. ACh contractile responses were similar in LZR and OZR. EFS-elicited contractions, however, were reduced in OZR bladder. Cannabinoid receptor agonists and antagonists failed to modify the DSM basal tension in LZR and OZR In LZR bladder, EFS responses were inhibited by ACEA and SER-601, CB1 and CB2 receptor agonists, respectively, these effects being reversed by ACEA plus the CB1 antagonist, AM-251 or SER-601 plus the CB2 antagonist, AM-630. In OZR bladder, the inhibitory action of ACEA on nerve-evoked contractions was diminished, whereas that SER-601 did not change EFS responses. These results suggest that a diminished function and expression of neuronal cannabinoid CB1 and CB2 receptors, as well as a lower nerve fiber density is involved in the impaired excitatory neurotransmission of the urinary bladder from the OZR.<br />Depto. de Enfermería<br />Fac. de Enfermería, Fisioterapia y Podología<br />TRUE<br />pub ) Array ( [Name] => Subject [Label] => Index Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%2261%22">61</searchLink><br /><searchLink fieldCode="DE" term="%22612%2E019%22">612.019</searchLink><br /><searchLink fieldCode="DE" term="%22Ciencias+Biomédicas%22">Ciencias Biomédicas</searchLink><br /><searchLink fieldCode="DE" term="%22Fisiología+veterinaria%22">Fisiología veterinaria</searchLink><br /><searchLink fieldCode="DE" term="%2224+Ciencias+de+la+Vida%22">24 Ciencias de la Vida</searchLink><br /><searchLink fieldCode="DE" term="%22journal+article%22">journal article</searchLink><br /><searchLink fieldCode="DE" term="%22VoR%22">VoR</searchLink> ) Array ( [Name] => URL [Label] => URL [Group] => URL [Data] => <externalLink term="https://hdl.handle.net/20.500.14352/97370">https://hdl.handle.net/20.500.14352/97370</externalLink><br /><externalLink term="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157424">https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157424</externalLink><br />PR6/13-18858 Universidad Complutense de Madrid ) Array ( [Name] => Avail [Label] => Availability [Group] => [Data] => Open access content. Open access content<br />Attribution 4.0 International<br />http://creativecommons.org/licenses/by/4.0<br />open access ) Array ( [Name] => Note [Label] => Note [Group] => [Data] => application/pdf<br />1932-6203<br />English ) Array ( [Name] => NumberOther [Label] => Other Numbers [Group] => [Data] => ESRCM oai:docta.ucm.es:20.500.14352/97370<br />Blaha I, Recio P, Martínez MP, López-Oliva ME, Ribeiro ASF, Agis-Torres Á, et al. (2016) Impaired Excitatory Neurotransmission in the Urinary Bladder from the Obese Zucker Rat: Role of Cannabinoid Receptors. PLoS ONE 11(6): e0157424. https://doi.org/10.1371/journal.pone.0157424<br />1932-6203<br />10.1371/journal.pone.0157424<br />1429623957 ) Array ( [Name] => OtherAuthors [Label] => Contributing Source [Group] => [Data] => REPOSITORIO E-PRINTS UNIVERSIDAD COMPLU<br />From OAIster®, provided by the OCLC Cooperative. ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsoai.on1429623957 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Impaired Excitatory Neurotransmission in the Urinary Bladder from the Obese Zucker Rat: Role of Cannabinoid Receptors
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Agustín Guerrero-Hernandez
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Blaha, Igor
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Recio Visedo, María Paz
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Martínez Sainz, María Del Pilar
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => López-Oliva Muñoz, María Elvira
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Fernandes Ribeiro, Ana Sofía
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Agis Torres, Ángel
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Martínez Gómez, Ana Cristina
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Benedito Castellote, Sara
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => García Sacristán, Albino
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Fernandes, Vítor S.
)
)
)
[11] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Hernández Rodríguez, Medardo Vicente
)
)
)
[12] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Agustín Guerrero-Hernandez
)
)
)
[13] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Blaha, Igor
)
)
)
[14] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Recio Visedo, María Paz
)
)
)
[15] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Martínez Sainz, María Del Pilar
)
)
)
[16] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => López-Oliva Muñoz, María Elvira
)
)
)
[17] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Fernandes Ribeiro, Ana Sofía
)
)
)
[18] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Agis Torres, Ángel
)
)
)
[19] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Martínez Gómez, Ana Cristina
)
)
)
[20] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Benedito Castellote, Sara
)
)
)
[21] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => García Sacristán, Albino
)
)
)
[22] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Fernandes, Vítor S.
)
)
)
[23] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Hernández Rodríguez, Medardo Vicente
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2024
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsoaiOpen
)
)
)
)
)
)
)
|
| IllustrationInfo |