Electronic Resource
Directed evolution of unspecific peroxygenase
| العنوان: | Directed evolution of unspecific peroxygenase |
|---|---|
| المؤلفون: | European Commission, Ministerio de Economía y Competitividad (España), Molina-Espeja, Patricia, Gómez de Santos, Patricia, Alcalde Galeote, Miguel |
| بيانات النشر: | Springer Nature 2017 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Unspecific peroxygenase (UPO) is a heme-thiolate peroxidase with mono(per)oxygenase activity for the selective oxyfunctionalization of C-H bonds. Fueled by catalytic concentrations of H2O2, which acts as both oxygen donor and as final electron acceptor, this stable, soluble and extracellular enzyme is a potential biocatalyst for dozens of transformations that are of considerable interest in organic synthesis. In this chapter we describe the main attributes of this versatile enzyme, while reflecting on the directed evolution campaigns recently followed in our laboratory that set out to enhance the functional expression of UPO in yeast and improve the activity, as well as approximating its properties to the required industrial standards. |
| مصطلحات الفهرس: | Unspecific peroxygenase, Directed evolution, Oxyfunctionalizations, Organic synthesis, Functional expression, Saccharomyces cerevisiae, Peroxidase activity, Peroxygenase activity, capítulo de libro |
| URL: | Sí info:eu-repo/grantAgreement/EC/FP7/613549 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-43407-R |
| الاتاحة: | Open access content. Open access content closedAccess |
| ملاحظة: | English |
| Other Numbers: | CTK oai:digital.csic.es:10261/165886 Directed Enzyme Evolution: Advances and Applications 5: 127-143 (2017) 978-3-319-50411-7 eBook ISBN 978-3-319-50413-1 10.1007/978-3-319-50413-1 1105217707 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1105217707 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |