التفاصيل البيبلوغرافية
| العنوان: |
Transgenní myši: Experimentální model pro studium akutní lymfoblastické leukemie |
| Alternate Title: |
Transgenic mouse: Experimental model for study of acute lymphoblastic leukemia. |
| المؤلفون: |
Kozáková, Jana |
| Thesis Advisors: |
Semecký, Vladimír, Lenčo, Juraj |
| سنة النشر: |
2008 |
| المجموعة: |
Czech ETDs |
| الوصف: |
The TEL and AML1 genes occur in a markedly high numer of different aberrations in heamatological malignancies. We studied TEL/AML hybride gene in transgenic mice model, translocation t(12;21)(p13;q22). This hybride gene was searched by PCR methode in 76 individuals. After, they were examined for presence of mRNA of fusion gen TEL/AML1 using RQ-RT-PCR. Compared the blood cell analysis of transgenic and non-transgenic mice, we searched difference in the erytrocyte, lymphocyte, trombocyte count and lymphocyte ratio in peripheral blood. The TEL/AML1 positive Acute lymphoblastic leukemia is B cell precursor leukemia, therefor we used cytofloumetric analysis for study the ratio of the B lymphocytes of transgenic and non-transgenic mice.Then, we observed the leukemic cell marker of pre-B lymphocytes (B220+ /CD43+ ). Presence of the pre-B lymphocytes in peripheral blood show incomplete differenciation and proliferation of B lymphocytes, cosequently leucemogenesis. |
| Original Identifier: |
oai:invenio.nusl.cz:292620 |
| نوع الوثيقة: |
masterThesis |
| اللغة: |
Czech |
| الاتاحة: |
http://www.nusl.cz/ntk/nusl-292620 |
| Rights: |
info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: |
edsndl.nusl.cz.oai.invenio.nusl.cz.292620 |
| قاعدة البيانات: |
Networked Digital Library of Theses & Dissertations |