Academic Journal
IFITM3 Interacts with the HBV/HDV Receptor NTCP and Modulates Virus Entry and Infection
| العنوان: | IFITM3 Interacts with the HBV/HDV Receptor NTCP and Modulates Virus Entry and Infection |
|---|---|
| المؤلفون: | Massimo Palatini, Simon Franz Müller, Michael Kirstgen, Silke Leiting, Felix Lehmann, Lena Soppa, Nora Goldmann, Christin Müller, Kira Alessandra Alicia Theresa Lowjaga, Jörg Alber, Giuliano Ciarimboli, John Ziebuhr, Dieter Glebe, Joachim Geyer |
| المصدر: | Viruses, Vol 14, Iss 4, p 727 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | HBV, HDV, infection, NTCP, IFITM3, virus entry, Microbiology, QR1-502 |
| الوصف: | The Na+/taurocholate co-transporting polypeptide (NTCP, gene symbol SLC10A1) is both a physiological bile acid transporter and the high-affinity hepatic receptor for the hepatitis B and D viruses (HBV/HDV). Virus entry via endocytosis of the virus/NTCP complex involves co-factors, but this process is not fully understood. As part of the innate immunity, interferon-induced transmembrane proteins (IFITM) 1–3 have been characterized as virus entry-restricting factors for many viruses. The present study identified IFITM3 as a novel protein–protein interaction (PPI) partner of NTCP based on membrane yeast-two hybrid and co-immunoprecipitation experiments. Surprisingly, IFITM3 knockdown significantly reduced in vitro HBV infection rates of NTCP-expressing HuH7 cells and primary human hepatocytes (PHHs). In addition, HuH7-NTCP cells showed significantly lower HDV infection rates, whereas infection with influenza A virus was increased. HBV-derived myr-preS1 peptide binding to HuH7-NTCP cells was intact even under IFITM3 knockdown, suggesting that IFITM3-mediated HBV/HDV infection enhancement occurs in a step subsequent to the viral attachment to NTCP. In conclusion, IFITM3 was identified as a novel NTCP co-factor that significantly affects in vitro infection with HBV and HDV in NTCP-expressing hepatoma cells and PHHs. While there is clear evidence for a direct PPI between IFITM3 and NTCP, the specific mechanism by which this PPI facilitates the infection process remains to be identified in future studies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 14040727 1999-4915 |
| Relation: | https://www.mdpi.com/1999-4915/14/4/727; https://doaj.org/toc/1999-4915 |
| DOI: | 10.3390/v14040727 |
| URL الوصول: | https://doaj.org/article/bcbcb0a170f44484a151b1e7d231ffdf |
| رقم الانضمام: | edsdoj.bcbcb0a170f44484a151b1e7d231ffdf |
| قاعدة البيانات: | Directory of Open Access Journals |
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