Academic Journal
Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
| العنوان: | Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway |
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| المؤلفون: | Okić-Đorđević Ivana, Kukolj Tamara, Obradović Hristina, Trivanović Drenka, Petrović Anđelija, Mojsilović Slavko, Miletić Maja, Bugarski Diana, Jauković Aleksandra |
| المصدر: | Archives of Biological Sciences, Vol 74, Iss 1, Pp 15-24 (2022) |
| بيانات النشر: | University of Belgrade, University of Novi Sad, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | interleukin-17, periodontal ligament mesenchymal stem cells (pdlmsc), urokinase-type plasminogen activator (upa), metalloproteinase (mmp), extracellular signal-regulated kinase (erk)1/2, mitogen-activated protein kinase (mapk) signaling pathway, Biology (General), QH301-705.5 |
| الوصف: | Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0354-4664 1821-4339 |
| Relation: | https://doaj.org/toc/0354-4664; https://doaj.org/toc/1821-4339 |
| DOI: | 10.2298/ABS210929048O |
| URL الوصول: | https://doaj.org/article/9a06f920423a410daa22347c6426ac20 |
| رقم الانضمام: | edsdoj.9a06f920423a410daa22347c6426ac20 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03544664 18214339 |
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| DOI: | 10.2298/ABS210929048O |