Academic Journal
Self-Assembly of Tail Tube Protein of Bacteriophage vB_EcoS_NBD2 into Extremely Long Polytubes in E. coli and S. cerevisiae
| العنوان: | Self-Assembly of Tail Tube Protein of Bacteriophage vB_EcoS_NBD2 into Extremely Long Polytubes in E. coli and S. cerevisiae |
|---|---|
| المؤلفون: | Aliona Špakova, Eugenijus Šimoliūnas, Raminta Batiuškaitė, Simonas Pajeda, Rolandas Meškys, Rasa Petraitytė-Burneikienė |
| المصدر: | Viruses, Vol 11, Iss 3, p 208 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | bacteriophage vB_EcoS_NBD2, tail tube protein, self-assembly, tubular structure, polytubes, stability, Saccharomyces cerevisiae, Escherichia coli, Microbiology, QR1-502 |
| الوصف: | Nucleotides, peptides and proteins serve as a scaffold material for self-assembling nanostructures. In this study, the production of siphovirus vB_EcoS_NBD2 (NBD2) recombinant tail tube protein gp39 reached approximately 33% and 27% of the total cell protein level in Escherichia coli and Saccharomyces cerevisiae expression systems, respectively. A simple purification protocol allowed us to produce a recombinant gp39 protein with 85%–90% purity. The yield of gp39 was 2.9 ± 0.36 mg/g of wet E. coli cells and 0.85 ± 0.33 mg/g for S. cerevisiae cells. The recombinant gp39 self-assembled into well-ordered tubular structures (polytubes) in vivo in the absence of other phage proteins. The diameter of these structures was the same as the diameter of the tail of phage NBD2 (~12 nm). The length of these structures varied from 0.1 µm to >3.95 µm, which is 23-fold the normal NBD2 tail length. Stability analysis demonstrated that the polytubes could withstand various chemical and physical conditions. These polytubes show the potential to be used as a nanomaterial in various fields of science. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1999-4915 11030208 |
| Relation: | http://www.mdpi.com/1999-4915/11/3/208; https://doaj.org/toc/1999-4915 |
| DOI: | 10.3390/v11030208 |
| URL الوصول: | https://doaj.org/article/dd9581b8c822443daeb58dae169f4433 |
| رقم الانضمام: | edsdoj.9581b8c822443daeb58dae169f4433 |
| قاعدة البيانات: | Directory of Open Access Journals |
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