Academic Journal
Maize Streak Virus Coat Protein Is Karyophyllic and Facilitates Nuclear Transport of Viral DNA
| العنوان: | Maize Streak Virus Coat Protein Is Karyophyllic and Facilitates Nuclear Transport of Viral DNA |
|---|---|
| المؤلفون: | H. Liu, M. I. Boulton, C. L. Thomas, D. A. M. Prior, K. J. Oparka, J. W. Davies |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 12, Iss 10, Pp 894-900 (1999) |
| بيانات النشر: | The American Phytopathological Society, 1999. |
| سنة النشر: | 1999 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | Transport of maize streak virus (MSV) DNA into the nucleus of host cells is essential for virus replication and the presence of virus particles in the nuclei of infected cells implies that coat protein (CP) must enter the nucleus. To see if CP is imported into the nucleus in the absence of other viral gene products, the MSV CP gene was expressed in insect cells with a baculovirus vector system, and also in tobacco protoplasts with a cauliflower mosaic virus (CaMV) 35S promoter-driven transient gene expression vector. Immunofluorescent staining showed that the CP accumulated in the nuclei of both insect and tobacco cells. Mutagenesis of a potential nuclear localization signal in the CP resulted in cytoplasmic accumulation of the mutant protein. We have shown previously that the CP binds to single-stranded (ss) and double-stranded (ds) viral DNA. To investigate if CP might also be involved in viral DNA nuclear transport, Escherichia coli-expressed CP, together with TOTO-1-labeled viral ss or ds DNA, was microinjected into maize and tobacco epidermal cells. Both ss and ds DNA moved into the nucleus when co-injected with the CP but not with E. coli proteins alone. These results suggest that, in addition to entering the nucleus where it is required for encapsidation of the viral ss DNA, the MSV CP facilitates the rapid transport of viral (ss or ds) DNA into the nucleus. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI.1999.12.10.894 |
| URL الوصول: | https://doaj.org/article/a67bda63a5ac4af180becaa662db8ac6 |
| رقم الانضمام: | edsdoj.67bda63a5ac4af180becaa662db8ac6 |
| قاعدة البيانات: | Directory of Open Access Journals |
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