Academic Journal

Photonic Dipstick Immunosensor to Detect Adulteration of Ewe, Goat, and Donkey Milk with Cow Milk through Bovine κ-Casein Detection

التفاصيل البيبلوغرافية
العنوان: Photonic Dipstick Immunosensor to Detect Adulteration of Ewe, Goat, and Donkey Milk with Cow Milk through Bovine κ-Casein Detection
المؤلفون: Dimitra Kourti, Michailia Angelopoulou, Eleni Makarona, Anastasios Economou, Panagiota Petrou, Konstantinos Misiakos, Sotirios Kakabakos
المصدر: Sensors, Vol 24, Iss 17, p 5688 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Chemical technology
مصطلحات موضوعية: photonic chip immunosensor, Mach–Zehnder interferometry, label-free detection, milk adulteration, bovine κ-casein, Chemical technology, TP1-1185
الوصف: The quality and authenticity of milk are of paramount importance. Cow milk is more allergenic and less nutritious than ewe, goat, or donkey milk, which are often adulterated with cow milk due to their seasonal availability and higher prices. In this work, a silicon photonic dipstick sensor accommodating two U-shaped Mach–Zehnder Interferometers (MZIs) was employed for the label-free detection of the adulteration of ewe, goat, and donkey milk with cow milk. One of the two MZIs of the chip was modified with bovine κ-casein, while the other was modified with bovine serum albumin to serve as a blank. All assay steps were performed by immersion of the chip side where the MZIs are positioned into the reagent solutions, leading to a photonic dipstick immunosensor. Thus, the chip was first immersed in a mixture of milk with anti-bovine κ-casein antibody and then in a secondary antibody solution for signal enhancement. A limit of detection of 0.05% v/v cow milk in ewe, goat, or donkey milk was achieved in 12 min using a 50-times diluted sample. This fast, sensitive, and simple assay, without the need for sample pre-processing, microfluidics, or pumps, makes the developed sensor ideal for the detection of milk adulteration at the point of need.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1424-8220
31963382
Relation: https://www.mdpi.com/1424-8220/24/17/5688; https://doaj.org/toc/1424-8220
DOI: 10.3390/s24175688
URL الوصول: https://doaj.org/article/e63592be8672401a9d3196338283d758
رقم الانضمام: edsdoj.63592be8672401a9d3196338283d758
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14248220
31963382
DOI:10.3390/s24175688