Academic Journal
Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: Post-Grafting Safety Characterization in Rats and Adult Pig
| العنوان: | Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: Post-Grafting Safety Characterization in Rats and Adult Pig |
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| المؤلفون: | Yoshiomi Kobayashi, Michiko Shigyo, Oleksandr Platoshyn, Silvia Marsala, Tomohisa Kato, Naoki Takamura, Kenji Yoshida, Akiyoshi Kishino, Mariana Bravo-Hernandez, Stefan Juhas, Jana Juhasova, Hana Studenovska, Vladimir Proks, Shawn P. Driscoll, Thomas D. Glenn, Samuel L. Pfaff, Joseph D. Ciacci, Martin Marsala |
| المصدر: | Cell Transplantation, Vol 32 (2023) |
| بيانات النشر: | SAGE Publishing, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Medicine |
| الوصف: | One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1555-3892 09636897 |
| Relation: | https://doaj.org/toc/1555-3892 |
| DOI: | 10.1177/09636897221107009 |
| URL الوصول: | https://doaj.org/article/53f0f5b81b344c76bd490c0f787e3315 |
| رقم الانضمام: | edsdoj.53f0f5b81b344c76bd490c0f787e3315 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15553892 09636897 |
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| DOI: | 10.1177/09636897221107009 |