Academic Journal
Fluorescence Turn-Off Ligand for Parallel G-Quadruplexes
| العنوان: | Fluorescence Turn-Off Ligand for Parallel G-Quadruplexes |
|---|---|
| المؤلفون: | Joanna Nowak-Karnowska, Agata Głuszyńska, Joanna Kosman, Anna Dembska |
| المصدر: | Molecules, Vol 29, Iss 16, p 3907 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | parallel G-quadruplexes, 9-methoxyluminarine, fluorescence, CD spectroscopy, UV–vis spectroscopy, Organic chemistry, QD241-441 |
| الوصف: | Parallel-stranded G-quadruplex structures are found to be common in the human promoter sequences. We tested highly fluorescent 9-methoxyluminarine ligand (9-MeLM) binding interactions with different parallel G-quadruplexes DNA by spectroscopic methods such as fluorescence and circular dichroism (CD) titration as well as UV melting profiles. The results showed that the studied 9-MeLM ligand interacted with the intramolecular parallel G-quadruplexes (G4s) with similar affinity. The binding constants of 9-methoxyluminarine with different parallel G4s were determined. The studies upon oligonucleotides with different flanking sequences on c-MYC G-quadruplex suggest that 9-methoxyluminarine may preferentially interact with 3′end of the c-MYC promoter. The high decrease in 9-MeLM ligand fluorescence upon binding to all tested G4s indicates that 9-methoxyluminarine molecule can be used as a selective fluorescence turn-off probe for parallel G-quadruplexes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1420-3049 |
| Relation: | https://www.mdpi.com/1420-3049/29/16/3907; https://doaj.org/toc/1420-3049 |
| DOI: | 10.3390/molecules29163907 |
| URL الوصول: | https://doaj.org/article/51fcda63ffc3477e959610b065f43013 |
| رقم الانضمام: | edsdoj.51fcda63ffc3477e959610b065f43013 |
| قاعدة البيانات: | Directory of Open Access Journals |
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