Academic Journal
Co-expression of thermophilic pectinases in a single host for cost-effective pectin bioconversion into D-galacturonic acid
| العنوان: | Co-expression of thermophilic pectinases in a single host for cost-effective pectin bioconversion into D-galacturonic acid |
|---|---|
| المؤلفون: | Carol N. Flores-Fernández, Max Cárdenas-Fernández, Gary J. Lye, John M. Ward |
| المصدر: | Frontiers in Catalysis, Vol 3 (2023) |
| بيانات النشر: | Frontiers Media S.A., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Chemistry |
| مصطلحات موضوعية: | enzymes co-expression, thermophilic pectinases, pectin, D-galacturonic acid, pectin methyl esterases, exo-polygalacturonases, Chemistry, QD1-999 |
| الوصف: | Co-expression of enzymes allow to produce multiple enzymes in a single host, representing a cost-effective alternative in biocatalytic processes which can be used for pectin bioconversion. Pectin-rich biomass is an abundant by-product from the fruit and sugar industries that is usually disposed in landfill or sold as a low value feedstock. The aim of this work was to co-express a thermophilic pectin methyl esterase (PME) and exo-polygalacturonases (exo-PGs) in a single host for pectin bioconversion into D-galacturonic acid (GalA) using different pectic substrates such as apple, citrus and sugar beet pectin. To achieve this, a PME from Bacillus licheniformis (BLI09) with either an exo-PG from Thermotoga maritima (TMA01) or from Bacillus licheniformis (BLI04) were cloned in pETDuet-1 and co-expressed in E. coli BL21 (DE3). Four co-expression plasmids containing both pectinases were constructed and factors such as the effect of the genes’ cloning order and their expression were evaluated. Co-expression constructs 3 and 4 (pETDuet-TMA01-BLI09 and pETDuet-BLI04-BLI09, respectively) showed better expression of both pectinases compared to co-expression constructs 1 and 2 (pETDuet-BLI09-TMA01 and pETDuet-BLI09-BLI04, respectively). Co-expression constructs 3 and 4 were the most efficient for pectin bioconversion into GalA reaching 3 and 2.5 mM GalA, respectively from apple and citrus pectin after 4 h reaction. In conclusion, this work demonstrates that the co-expression of pectinases can potentially contribute to reduce the cost associated to their production and purification as well as to increase their applicability for exploiting pectin-rich biomass to obtain bio-based chemicals. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2673-7841 |
| Relation: | https://www.frontiersin.org/articles/10.3389/fctls.2023.1112154/full; https://doaj.org/toc/2673-7841 |
| DOI: | 10.3389/fctls.2023.1112154 |
| URL الوصول: | https://doaj.org/article/49ff3edcc92947c2846d35b9636b4e6d |
| رقم الانضمام: | edsdoj.49ff3edcc92947c2846d35b9636b4e6d |
| قاعدة البيانات: | Directory of Open Access Journals |
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