Academic Journal
Anaerobic RSH-dependent tellurite reduction contributes to Escherichia coli tolerance against tellurite
| العنوان: | Anaerobic RSH-dependent tellurite reduction contributes to Escherichia coli tolerance against tellurite |
|---|---|
| المؤلفون: | P. Muñoz-Diaz, K. Jiménez, R. Luraschi, F. Cornejo, M. Figueroa, C. Vera, A. Rivas-Pardo, J. M. Sandoval, C. Vásquez, F. Arenas |
| المصدر: | Biological Research, Vol 55, Iss 1, Pp 1-10 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Tellurite reduction, Escherichia coli, Glutathione, RSH, Biology (General), QH301-705.5 |
| الوصف: | Abstract Background Tellurium is a rare metalloid that exerts high toxicity on cells, especially on bacteria, partly due to reactive oxygen species (ROS) generation. Moreover, it has also been observed that tellurite can target free cell thiols groups (RSH) (i.e. reduced glutathione (GSH)), enhancing the cellular redox imbalance. Additionally, in vitro experiments have suggested that several enzymes can reduce tellurite (IV) to its elemental form (0); where RSH present on their active sites may be responsible for the process. Nevertheless, the mechanisms implemented by bacteria for tellurite reduction and its role in resistance have not been evaluated in vivo. Results This work shows that tellurite reduction to elemental tellurium is increased under anaerobic conditions in E. coli cells. The in vivo tellurite reduction is related to the intracellular concentration of total RSH, in the presence and absence of oxygen. This metabolization of tellurite directly contributes to the resistance of the bacteria to the oxyanion. Conclusions We demonstrated that in vivo tellurite reduction is related to the intracellular thiol concentration, i.e. large availability of cellular RSH groups, results in a more significant reduction of tellurite. Furthermore, we observed that, when the bacterium exhibits less resistance to the oxyanion, a decreased tellurite reduction was seen, affecting the growth fitness. Together, these results let us propose that tellurite reduction and the intracellular RSH content are related to the oxyanion bacterial resistance, this tripartite mechanism in an oxygen-independent anaerobic process. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0717-6287 |
| Relation: | https://doaj.org/toc/0717-6287 |
| DOI: | 10.1186/s40659-022-00383-5 |
| URL الوصول: | https://doaj.org/article/326f5523c6714a73bce60bfb3293b89c |
| رقم الانضمام: | edsdoj.326f5523c6714a73bce60bfb3293b89c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edsdoj Directory of Open Access Journals edsdoj.326f5523c6714a73bce60bfb3293b89c 967 3 Academic Journal academicJournal 967.350158691406 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.326f5523c6714a73bce60bfb3293b89c&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doaj.org/article/326f5523c6714a73bce60bfb3293b89c [Name] => EDS - DOAJ [Category] => fullText [Text] => View record in DOAJ [MouseOverText] => View record in DOAJ ) [1] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edsdoj&genre=article&issn=07176287&ISBN=&volume=55&issue=1&date=20220301&spage=1&pages=1-10&title=Biological Research&atitle=Anaerobic%20RSH-dependent%20tellurite%20reduction%20contributes%20to%20Escherichia%20coli%20tolerance%20against%20tellurite&id=DOI:10.1186/s40659-022-00383-5 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Anaerobic RSH-dependent tellurite reduction contributes to Escherichia coli tolerance against tellurite
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22P%2E+Muñoz-Diaz%22">P. Muñoz-Diaz</searchLink><br /><searchLink fieldCode="AR" term="%22K%2E+Jiménez%22">K. Jiménez</searchLink><br /><searchLink fieldCode="AR" term="%22R%2E+Luraschi%22">R. Luraschi</searchLink><br /><searchLink fieldCode="AR" term="%22F%2E+Cornejo%22">F. Cornejo</searchLink><br /><searchLink fieldCode="AR" term="%22M%2E+Figueroa%22">M. Figueroa</searchLink><br /><searchLink fieldCode="AR" term="%22C%2E+Vera%22">C. Vera</searchLink><br /><searchLink fieldCode="AR" term="%22A%2E+Rivas-Pardo%22">A. Rivas-Pardo</searchLink><br /><searchLink fieldCode="AR" term="%22J%2E+M%2E+Sandoval%22">J. M. Sandoval</searchLink><br /><searchLink fieldCode="AR" term="%22C%2E+Vásquez%22">C. Vásquez</searchLink><br /><searchLink fieldCode="AR" term="%22F%2E+Arenas%22">F. Arenas</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Biological Research, Vol 55, Iss 1, Pp 1-10 (2022) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => BMC, 2022. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2022 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => LCC:Biology (General) ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Tellurite+reduction%22">Tellurite reduction</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Glutathione%22">Glutathione</searchLink><br /><searchLink fieldCode="DE" term="%22RSH%22">RSH</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Abstract Background Tellurium is a rare metalloid that exerts high toxicity on cells, especially on bacteria, partly due to reactive oxygen species (ROS) generation. Moreover, it has also been observed that tellurite can target free cell thiols groups (RSH) (i.e. reduced glutathione (GSH)), enhancing the cellular redox imbalance. Additionally, in vitro experiments have suggested that several enzymes can reduce tellurite (IV) to its elemental form (0); where RSH present on their active sites may be responsible for the process. Nevertheless, the mechanisms implemented by bacteria for tellurite reduction and its role in resistance have not been evaluated in vivo. Results This work shows that tellurite reduction to elemental tellurium is increased under anaerobic conditions in E. coli cells. The in vivo tellurite reduction is related to the intracellular concentration of total RSH, in the presence and absence of oxygen. This metabolization of tellurite directly contributes to the resistance of the bacteria to the oxyanion. Conclusions We demonstrated that in vivo tellurite reduction is related to the intracellular thiol concentration, i.e. large availability of cellular RSH groups, results in a more significant reduction of tellurite. Furthermore, we observed that, when the bacterium exhibits less resistance to the oxyanion, a decreased tellurite reduction was seen, affecting the growth fitness. Together, these results let us propose that tellurite reduction and the intracellular RSH content are related to the oxyanion bacterial resistance, this tripartite mechanism in an oxygen-independent anaerobic process. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => electronic resource ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0717-6287 ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://doaj.org/toc/0717-6287 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1186/s40659-022-00383-5 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://doaj.org/article/326f5523c6714a73bce60bfb3293b89c" linkWindow="_blank">https://doaj.org/article/326f5523c6714a73bce60bfb3293b89c</link> ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsdoj.326f5523c6714a73bce60bfb3293b89c ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1186/s40659-022-00383-5
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 10
[StartPage] => 1
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Tellurite reduction
[Type] => general
)
[1] => Array
(
[SubjectFull] => Escherichia coli
[Type] => general
)
[2] => Array
(
[SubjectFull] => Glutathione
[Type] => general
)
[3] => Array
(
[SubjectFull] => RSH
[Type] => general
)
[4] => Array
(
[SubjectFull] => Biology (General)
[Type] => general
)
[5] => Array
(
[SubjectFull] => QH301-705.5
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Anaerobic RSH-dependent tellurite reduction contributes to Escherichia coli tolerance against tellurite
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => P. Muñoz-Diaz
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => K. Jiménez
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => R. Luraschi
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => F. Cornejo
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => M. Figueroa
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => C. Vera
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => A. Rivas-Pardo
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => J. M. Sandoval
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => C. Vásquez
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => F. Arenas
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 03
[Type] => published
[Y] => 2022
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 07176287
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 55
)
[1] => Array
(
[Type] => issue
[Value] => 1
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Biological Research
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |