Academic Journal
Efficient induction of functional ameloblasts from human keratinocyte stem cells
| العنوان: | Efficient induction of functional ameloblasts from human keratinocyte stem cells |
|---|---|
| المؤلفون: | Xuefeng Hu, Jyh-Wei Lee, Xi Zheng, Junhua Zhang, Xin Lin, Yingnan Song, Bingmei Wang, Xiaoxiao Hu, Hao-Hueng Chang, Yiping Chen, Chun-Pin Lin, Yanding Zhang |
| المصدر: | Stem Cell Research & Therapy, Vol 9, Iss 1, Pp 1-13 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Fibroblast growth factor 8, Sonic hedgehog, Keratinocyte stem cells, Ameloblast, Enamel, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study. Methods Cultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13.5 mouse dental mesenchyme with implantation of FGF8 and SHH-soaked agarose beads into reconstructed chimeric tooth germs. Recombinant tooth germs were subjected to kidney capsule culture in nude mice. Harvested samples at various time points were processed for histological, immunohistochemical, TUNEL, and western blot analysis. Scanning electronic microscopy and a nanoindentation test were further employed to analyze the prism running pattern and mechanical properties of the regenerated enamel. Results Treatment of hKSCs with both FGF8 and SHH prior to tissue recombination greatly enhanced the rate of tooth-like structure formation to about 70%. FGF8 and SHH dramatically enhanced stemness of cultured hKSCs. Scanning electron microscopic analysis revealed the running pattern of intact prisms of regenerated enamel is similar to that of human enamel. The nanoindentation test indicated that, although much softer than human child and adult mouse enamel, mechanical properties of the regenerated enamel improved as the culture time was extended. Conclusions Application of FGF8 and SHH proteins in cultured hKSCs improves stemness but does not facilitate odontogenic fate of hKSCs, resulting in an enhanced efficiency of ameloblastic differentiation of hKSCs and tooth formation in human–mouse chimeric tooth germs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | http://link.springer.com/article/10.1186/s13287-018-0822-4; https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-018-0822-4 |
| URL الوصول: | https://doaj.org/article/a24a887fec28440685191fe3160a475a |
| رقم الانضمام: | edsdoj.24a887fec28440685191fe3160a475a |
| قاعدة البيانات: | Directory of Open Access Journals |
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