Academic Journal
Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk
| العنوان: | Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk |
|---|---|
| المؤلفون: | Mahmoud K. Eldahshoury, Ian P. Hurley |
| المصدر: | Journal of Dairy Science, Vol 106, Iss 9, Pp 5908-5915 (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Dairy processing. Dairy products |
| مصطلحات موضوعية: | human breast milk adulteration, sandwich ELISA, IgG, food adulteration, Dairy processing. Dairy products, SF250.5-275, Dairying, SF221-250 |
| الوصف: | ABSTRACT: The demand for commercially available human breast milk has significantly increased in recent years. For various reasons, a significant amount of commercially available human breast milk is being adulterated with other types of milk. This fraudulent practice poses a threat to consumers' health due to potential adulterants such as cow milk, which may put the infant at risk due to intolerance or allergy. A direct sandwich anti-bovine IgG ELISA has been developed for the sensitive and specific detection of cow milk in adulterated human breast milk. This assay uses polyclonal anti-bovine IgG antibody as a capture antibody and monoclonal anti-bovine IgG-alkaline phosphatase antibody as a detection antibody. Once optimized, the assay was found to be highly sensitive, and specific to bovine IgG. The assay had no significant cross-reaction with human breast milk, indicating that it was highly specific. The anti-bovine IgG ELISA was able to detect the presence of cow milk in adulterated human breast milk with a detection limit of 0.001% cow milk. The developed assay was highly reproducible (coefficient of variation |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0022-0302 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0022030223003922; https://doaj.org/toc/0022-0302 |
| DOI: | 10.3168/jds.2022-22589 |
| URL الوصول: | https://doaj.org/article/12d300bef6924ad3be58ed93a18563b9 |
| رقم الانضمام: | edsdoj.12d300bef6924ad3be58ed93a18563b9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder كن أول من يترك تعليقا!