Academic Journal
Global Run-On sequencing to measure nascent transcription in C. elegans
| العنوان: | Global Run-On sequencing to measure nascent transcription in C. elegans |
|---|---|
| المؤلفون: | Piergiuseppe Quarato, Germano Cecere |
| المصدر: | STAR Protocols, Vol 2, Iss 4, Pp 100991- (2021) |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science (General) |
| مصطلحات موضوعية: | Cell separation/fractionation, Gene Expression, Genomics, Model Organisms, Molecular Biology, RNAseq, Science (General), Q1-390 |
| الوصف: | Summary: Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to the nematode Caenorhabditis elegans to measure transcription from embryos and adult worms. Here, we provide a detailed overview of the protocol highlighting the critical steps for generating successful libraries.For complete details on the use and execution of this protocol, please refer to Quarato et al. (2021). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2666-1667 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666166721006973; https://doaj.org/toc/2666-1667 |
| DOI: | 10.1016/j.xpro.2021.100991 |
| URL الوصول: | https://doaj.org/article/0abc15f9e28d4468a27c16d52ee9151f |
| رقم الانضمام: | edsdoj.0abc15f9e28d4468a27c16d52ee9151f |
| قاعدة البيانات: | Directory of Open Access Journals |
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