Academic Journal
The Prozone Effect Accounts for the Paradoxical Function of the Cdk-Binding Protein Suc1/Cks
| العنوان: | The Prozone Effect Accounts for the Paradoxical Function of the Cdk-Binding Protein Suc1/Cks |
|---|---|
| المؤلفون: | Sang Hoon Ha, Sun Young Kim, James E. Ferrell Jr. |
| المصدر: | Cell Reports, Vol 14, Iss 6, Pp 1408-1421 (2016) |
| بيانات النشر: | Elsevier, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | Previous work has shown that Suc1/Cks proteins can promote the hyperphosphorylation of primed Cdk1 substrates through the formation of ternary Cdk1-Cks-phosphosubstrate complexes. This raises the possibility that Cks proteins might be able to both facilitate and interfere with hyperphosphorylation through a mechanism analogous to the prozone effect in antigen-antibody interactions, with substoichiometric Cks promoting the formation of Cdk1-Cks-phosphosubstrate complexes and suprastoichiometric Cks instead promoting the formation of Cdk1-Cks and Cks-phosphosubstrate complexes. We tested this hypothesis through a combination of theory, proof-of-principle experiments with oligonucleotide annealing, and experiments on the interaction of Xenopus cyclin B1-Cdk1-Cks2 with Wee1A in vitro and in Xenopus extracts. Our findings help explain why both Cks under-expression and overexpression interfere with cell-cycle progression and provide insight into the regulation of the Cdk1 system. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2211-1247 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2211124716000541; https://doaj.org/toc/2211-1247 |
| DOI: | 10.1016/j.celrep.2016.01.033 |
| URL الوصول: | https://doaj.org/article/07fb7963e10c4e8bb3354ac4b0f0a11a |
| رقم الانضمام: | edsdoj.07fb7963e10c4e8bb3354ac4b0f0a11a |
| قاعدة البيانات: | Directory of Open Access Journals |
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