Academic Journal
Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
| العنوان: | Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices |
|---|---|
| المؤلفون: | Rise Akasaka, Masashi Ozawa, Yuji Nashimoto, Kosuke Ino, Hitoshi Shiku |
| المصدر: | Micromachines, Vol 12, Iss 12, p 1491 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Mechanical engineering and machinery |
| مصطلحات موضوعية: | vascular vessel model, ion conductance assay, electrochemical device, perfusability assay, microfluidic device, Mechanical engineering and machinery, TJ1-1570 |
| الوصف: | We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial cells (HUVECs). Fibroblasts were cultured in the other channels to improve the vascular network formation. To form vessel structures bridging the center channel, HUVEC monolayers were prepared on both side walls of the gel. During the culture, the HUVECs migrated from the monolayer and connected to the HUVECs in the gel, and vascular vessels formed, resulting in successful perfusion between the channels after culturing for 3–5 d. To evaluate perfusion without microscopic imaging, Ag/AgCl wires were inserted into the channels, and ion currents were obtained to measure the ion conductance between the channels separated by the HUVEC monolayers. As the HUVEC monolayers blocked the ion current flow, the ion currents were low before vessel formation. In contrast, ion currents increased after vessel formation because of creation of ion current paths. Thus, the observed ion currents were correlated with the perfusability of the vessels, indicating that they can be used as indicators of perfusion during vessel formation in microfluidic devices. The developed methodology will be used for drug screening using organs-on-a-chip containing vascular vessels. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2072-666X |
| Relation: | https://www.mdpi.com/2072-666X/12/12/1491; https://doaj.org/toc/2072-666X |
| DOI: | 10.3390/mi12121491 |
| URL الوصول: | https://doaj.org/article/014afed5baea407481d78f3c6cae17d9 |
| رقم الانضمام: | edsdoj.014afed5baea407481d78f3c6cae17d9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edsdoj Directory of Open Access Journals edsdoj.014afed5baea407481d78f3c6cae17d9 907 3 Academic Journal academicJournal 906.677795410156 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.014afed5baea407481d78f3c6cae17d9&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doaj.org/article/014afed5baea407481d78f3c6cae17d9 [Name] => EDS - DOAJ [Category] => fullText [Text] => View record in DOAJ [MouseOverText] => View record in DOAJ ) [1] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edsdoj&genre=article&issn=2072666X&ISBN=&volume=12&issue=12&date=20211101&spage=1491&pages=1491-1491&title=Micromachines&atitle=Ion%20Conductance-Based%20Perfusability%20Assay%20of%20Vascular%20Vessel%20Models%20in%20Microfluidic%20Devices&id=DOI:10.3390/mi12121491 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Rise+Akasaka%22">Rise Akasaka</searchLink><br /><searchLink fieldCode="AR" term="%22Masashi+Ozawa%22">Masashi Ozawa</searchLink><br /><searchLink fieldCode="AR" term="%22Yuji+Nashimoto%22">Yuji Nashimoto</searchLink><br /><searchLink fieldCode="AR" term="%22Kosuke+Ino%22">Kosuke Ino</searchLink><br /><searchLink fieldCode="AR" term="%22Hitoshi+Shiku%22">Hitoshi Shiku</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Micromachines, Vol 12, Iss 12, p 1491 (2021) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => MDPI AG, 2021. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2021 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => LCC:Mechanical engineering and machinery ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22vascular+vessel+model%22">vascular vessel model</searchLink><br /><searchLink fieldCode="DE" term="%22ion+conductance+assay%22">ion conductance assay</searchLink><br /><searchLink fieldCode="DE" term="%22electrochemical+device%22">electrochemical device</searchLink><br /><searchLink fieldCode="DE" term="%22perfusability+assay%22">perfusability assay</searchLink><br /><searchLink fieldCode="DE" term="%22microfluidic+device%22">microfluidic device</searchLink><br /><searchLink fieldCode="DE" term="%22Mechanical+engineering+and+machinery%22">Mechanical engineering and machinery</searchLink><br /><searchLink fieldCode="DE" term="%22TJ1-1570%22">TJ1-1570</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial cells (HUVECs). Fibroblasts were cultured in the other channels to improve the vascular network formation. To form vessel structures bridging the center channel, HUVEC monolayers were prepared on both side walls of the gel. During the culture, the HUVECs migrated from the monolayer and connected to the HUVECs in the gel, and vascular vessels formed, resulting in successful perfusion between the channels after culturing for 3–5 d. To evaluate perfusion without microscopic imaging, Ag/AgCl wires were inserted into the channels, and ion currents were obtained to measure the ion conductance between the channels separated by the HUVEC monolayers. As the HUVEC monolayers blocked the ion current flow, the ion currents were low before vessel formation. In contrast, ion currents increased after vessel formation because of creation of ion current paths. Thus, the observed ion currents were correlated with the perfusability of the vessels, indicating that they can be used as indicators of perfusion during vessel formation in microfluidic devices. The developed methodology will be used for drug screening using organs-on-a-chip containing vascular vessels. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => electronic resource ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 2072-666X ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://www.mdpi.com/2072-666X/12/12/1491; https://doaj.org/toc/2072-666X ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.3390/mi12121491 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://doaj.org/article/014afed5baea407481d78f3c6cae17d9" linkWindow="_blank">https://doaj.org/article/014afed5baea407481d78f3c6cae17d9</link> ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsdoj.014afed5baea407481d78f3c6cae17d9 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3390/mi12121491
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 1
[StartPage] => 1491
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => vascular vessel model
[Type] => general
)
[1] => Array
(
[SubjectFull] => ion conductance assay
[Type] => general
)
[2] => Array
(
[SubjectFull] => electrochemical device
[Type] => general
)
[3] => Array
(
[SubjectFull] => perfusability assay
[Type] => general
)
[4] => Array
(
[SubjectFull] => microfluidic device
[Type] => general
)
[5] => Array
(
[SubjectFull] => Mechanical engineering and machinery
[Type] => general
)
[6] => Array
(
[SubjectFull] => TJ1-1570
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Rise Akasaka
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Masashi Ozawa
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yuji Nashimoto
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Kosuke Ino
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Hitoshi Shiku
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 11
[Type] => published
[Y] => 2021
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 2072666X
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 12
)
[1] => Array
(
[Type] => issue
[Value] => 12
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Micromachines
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |