Academic Journal
أعرض في EDS

Patogenicidad dérmica de un aislamiento autóctono del hongo entomopatógeno beauveria bassiana en ratones ; Dermal pathogenicity of an autochthonous isolate of entomopathogenic fungus beauveria bassiana in mice

التفاصيل البيبلوغرافية
العنوان: Patogenicidad dérmica de un aislamiento autóctono del hongo entomopatógeno beauveria bassiana en ratones ; Dermal pathogenicity of an autochthonous isolate of entomopathogenic fungus beauveria bassiana in mice
المؤلفون: Acosta Quintero, María Eugenia, Cazorla, Dalmiro, Eduarte Pereira, Giovanny, Morales Moreno, Pedro
سنة النشر: 2012
المجموعة: Universidad de Los Andes, Mérida, Venezuela: SaberULA
مصطلحات موضوعية: Beauveria bassiana, Toxicidad, Patogenicidad, Ratones, Infectividad, Universidad del Zulia (LUZ), Universidad de Los Andes (ULA), Toxicity, Pathogenicity, Revista Científica, Revista Científica: Medicina Veterinaria, Medio Ambiente, Revistas
الوصف: El objetivo de este trabajo fue evaluar a dosis de 1x109 conidias/ mL por vía dérmica en ratones albinos NMRI (machos y hembras), la patogenicidad del aislamiento autóctono LF14 de Beauveria bassiana (Fungi: Ascomycota). Esta especie posee una elevada virulencia hacia Rhodnius prolixus y Triatoma maculata (Triatominae), vectores de la enfermedad de Chagas en Venezuela. Se realizaron observaciones clínicas y de comportamiento diarias y se registró el peso corporal. Asimismo, se estimó el aclaramiento fúngico mediante examen directo y cultivo de muestras de piel y de infectividad con la toma de muestras de tejidos de varios órganos, incluyendo un estudio histopatológico durante las necropsias a los 3; 7 y 14 días después de la inoculación. No ocurrieron muertes ni alteraciones clínico-patológicas discernibles, siendo el tipo de comportamiento activonormal en el 100% de los casos. El peso promedio corporal ganado, tanto en los animales expuestos a LF14 B. bassiana como los controles, se incrementó con el tiempo, siendo sólo las diferencias entre sexos estadísticamente significativas (F=42,84; g.l.=1; P<0,00001). Se obtuvieron conidias viables sin germinar a las 24 horas después de la inoculación, lo que sugiere que no hubo germinación fúngica en la región dérmica. No se detectaron anormalidades en la inspección anatomopatológico ni conidias germinadas en ninguno de los órganos estudiados, sin ninguna reacción tisular patológica, sugiriendo que no existe evidencia de multiplicación fúngica. Se concluyó que aún a una dosis fúngica elevada, el aislamiento LF14 de B. bassiana no es patogénico por vía dérmica en ratones albinos NMRI. ; The purpose of this study was to evaluate with doses of 1x109 conidia/mL dermically administered in white mice (NMRI strain) (males and females), pathogenicity of a native isolate LF14 of Beauveria bassiana (Fungi: Ascomycota). This species is highly virulent to Rhodnius prolixus and Triatoma maculata (Triatominae), vectors of the Chagas disease in Venezuela. Clinical and behavioural evaluations were done daily, as well as weight of the mice. Likewise, clearance of fungus of the skin was estimated by direct examination and culturing, and infectivity by performing mycological and histopathological test after necropsies at 3, 7 and 14 days post-inoculation. Animals no showed discernible pathological changes or died, with 100% active-normal behaviour. In all the mice groups, including control ones, there was an increasing in the mean weight gained through time, with statistically significance difference between the sexes (F=53,2; g.l.=1; P<0,0000). Nongerminated conidia viable were observed 24 hours after inoculating fungus, suggesting there was no germination into dermal region. Anatomopathological changes or germinated conidia were not detected in analysed organs, with normal tissue reactions, suggesting no evidence for fungal multiplication. It can be concluded that even to a high dose dermically administered, isolate LF14, B. bassiana is non pathogenic in white mice (NMRI strain). ; 477 - 483 ; lutzomyia@hotmail.com ; Bimestral
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: Spanish; Castilian
تدمد: 0798-2259
Relation: http://www.saber.ula.ve/handle/123456789/34369; 199102ZU46
الاتاحة: http://www.saber.ula.ve/handle/123456789/34369
Rights: info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-sa/3.0/ve/
رقم الانضمام: edsbas.FE14BD2E
قاعدة البيانات: BASE
ResultId 1
Header edsbas
BASE
edsbas.FE14BD2E
844
3
Academic Journal
academicJournal
843.984313964844
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.FE14BD2E&custid=s6537998&authtype=sso
FullText Array ( [Availability] => 0 )
Array ( [0] => Array ( [Url] => http://www.saber.ula.ve/handle/123456789/34369# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) )
Items Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Patogenicidad d&#233;rmica de un aislamiento aut&#243;ctono del hongo entomopat&#243;geno beauveria bassiana en ratones ; Dermal pathogenicity of an autochthonous isolate of entomopathogenic fungus beauveria bassiana in mice )
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Acosta+Quintero%2C+Mar&#237;a+Eugenia%22&quot;&gt;Acosta Quintero, Mar&#237;a Eugenia&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cazorla%2C+Dalmiro%22&quot;&gt;Cazorla, Dalmiro&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Eduarte+Pereira%2C+Giovanny%22&quot;&gt;Eduarte Pereira, Giovanny&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Morales+Moreno%2C+Pedro%22&quot;&gt;Morales Moreno, Pedro&lt;/searchLink&gt; )
Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2012 )
Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Universidad de Los Andes, M&#233;rida, Venezuela: SaberULA )
Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Beauveria+bassiana%22&quot;&gt;Beauveria bassiana&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Toxicidad%22&quot;&gt;Toxicidad&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Patogenicidad%22&quot;&gt;Patogenicidad&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Ratones%22&quot;&gt;Ratones&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Infectividad%22&quot;&gt;Infectividad&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Universidad+del+Zulia+%28LUZ%29%22&quot;&gt;Universidad del Zulia (LUZ)&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Universidad+de+Los+Andes+%28ULA%29%22&quot;&gt;Universidad de Los Andes (ULA)&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Toxicity%22&quot;&gt;Toxicity&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Pathogenicity%22&quot;&gt;Pathogenicity&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Revista+Cient&#237;fica%22&quot;&gt;Revista Cient&#237;fica&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Revista+Cient&#237;fica%3A+Medicina+Veterinaria%22&quot;&gt;Revista Cient&#237;fica: Medicina Veterinaria&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Medio+Ambiente%22&quot;&gt;Medio Ambiente&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Revistas%22&quot;&gt;Revistas&lt;/searchLink&gt; )
Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => El objetivo de este trabajo fue evaluar a dosis de 1x109 conidias/ mL por v&#237;a d&#233;rmica en ratones albinos NMRI (machos y hembras), la patogenicidad del aislamiento aut&#243;ctono LF14 de Beauveria bassiana (Fungi: Ascomycota). Esta especie posee una elevada virulencia hacia Rhodnius prolixus y Triatoma maculata (Triatominae), vectores de la enfermedad de Chagas en Venezuela. Se realizaron observaciones cl&#237;nicas y de comportamiento diarias y se registr&#243; el peso corporal. Asimismo, se estim&#243; el aclaramiento f&#250;ngico mediante examen directo y cultivo de muestras de piel y de infectividad con la toma de muestras de tejidos de varios &#243;rganos, incluyendo un estudio histopatol&#243;gico durante las necropsias a los 3; 7 y 14 d&#237;as despu&#233;s de la inoculaci&#243;n. No ocurrieron muertes ni alteraciones cl&#237;nico-patol&#243;gicas discernibles, siendo el tipo de comportamiento activonormal en el 100% de los casos. El peso promedio corporal ganado, tanto en los animales expuestos a LF14 B. bassiana como los controles, se increment&#243; con el tiempo, siendo s&#243;lo las diferencias entre sexos estad&#237;sticamente significativas (F=42,84; g.l.=1; P&lt;0,00001). Se obtuvieron conidias viables sin germinar a las 24 horas despu&#233;s de la inoculaci&#243;n, lo que sugiere que no hubo germinaci&#243;n f&#250;ngica en la regi&#243;n d&#233;rmica. No se detectaron anormalidades en la inspecci&#243;n anatomopatol&#243;gico ni conidias germinadas en ninguno de los &#243;rganos estudiados, sin ninguna reacci&#243;n tisular patol&#243;gica, sugiriendo que no existe evidencia de multiplicaci&#243;n f&#250;ngica. Se concluy&#243; que a&#250;n a una dosis f&#250;ngica elevada, el aislamiento LF14 de B. bassiana no es patog&#233;nico por v&#237;a d&#233;rmica en ratones albinos NMRI. ; The purpose of this study was to evaluate with doses of 1x109 conidia/mL dermically administered in white mice (NMRI strain) (males and females), pathogenicity of a native isolate LF14 of Beauveria bassiana (Fungi: Ascomycota). This species is highly virulent to Rhodnius prolixus and Triatoma maculata (Triatominae), vectors of the Chagas disease in Venezuela. Clinical and behavioural evaluations were done daily, as well as weight of the mice. Likewise, clearance of fungus of the skin was estimated by direct examination and culturing, and infectivity by performing mycological and histopathological test after necropsies at 3, 7 and 14 days post-inoculation. Animals no showed discernible pathological changes or died, with 100% active-normal behaviour. In all the mice groups, including control ones, there was an increasing in the mean weight gained through time, with statistically significance difference between the sexes (F=53,2; g.l.=1; P&lt;0,0000). Nongerminated conidia viable were observed 24 hours after inoculating fungus, suggesting there was no germination into dermal region. Anatomopathological changes or germinated conidia were not detected in analysed organs, with normal tissue reactions, suggesting no evidence for fungal multiplication. It can be concluded that even to a high dose dermically administered, isolate LF14, B. bassiana is non pathogenic in white mice (NMRI strain). ; 477 - 483 ; lutzomyia@hotmail.com ; Bimestral )
Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper )
Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => application/pdf )
Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => Spanish; Castilian )
Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0798-2259 )
Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => http://www.saber.ula.ve/handle/123456789/34369; 199102ZU46 )
Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => http://www.saber.ula.ve/handle/123456789/34369 )
Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-sa/3.0/ve/ )
Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.FE14BD2E )
RecordInfo Array ( [BibEntity] => Array ( [Languages] => Array ( [0] => Array ( [Text] => Spanish; Castilian ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => Beauveria bassiana [Type] => general ) [1] => Array ( [SubjectFull] => Toxicidad [Type] => general ) [2] => Array ( [SubjectFull] => Patogenicidad [Type] => general ) [3] => Array ( [SubjectFull] => Ratones [Type] => general ) [4] => Array ( [SubjectFull] => Infectividad [Type] => general ) [5] => Array ( [SubjectFull] => Universidad del Zulia (LUZ) [Type] => general ) [6] => Array ( [SubjectFull] => Universidad de Los Andes (ULA) [Type] => general ) [7] => Array ( [SubjectFull] => Toxicity [Type] => general ) [8] => Array ( [SubjectFull] => Pathogenicity [Type] => general ) [9] => Array ( [SubjectFull] => Revista Científica [Type] => general ) [10] => Array ( [SubjectFull] => Revista Científica: Medicina Veterinaria [Type] => general ) [11] => Array ( [SubjectFull] => Medio Ambiente [Type] => general ) [12] => Array ( [SubjectFull] => Revistas [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Patogenicidad dérmica de un aislamiento autóctono del hongo entomopatógeno beauveria bassiana en ratones ; Dermal pathogenicity of an autochthonous isolate of entomopathogenic fungus beauveria bassiana in mice [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Acosta Quintero, María Eugenia ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Cazorla, Dalmiro ) ) ) [2] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Eduarte Pereira, Giovanny ) ) ) [3] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Morales Moreno, Pedro ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 01 [M] => 01 [Type] => published [Y] => 2012 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-print [Value] => 07982259 ) [1] => Array ( [Type] => issn-locals [Value] => edsbas ) [2] => Array ( [Type] => issn-locals [Value] => edsbas.oa ) ) ) ) ) ) )
IllustrationInfo