Dissertation/ Thesis
Effects of low-dose bisphenol A on transcriptional expression of breast cancer cell lines, tumor immunity, and cancer progression ; 저용량 비스페놀A 노출이 유방암 세포의 전사체 발현, 종양면역 및 암 진행에 미치는 영향에 관한 연구
| العنوان: | Effects of low-dose bisphenol A on transcriptional expression of breast cancer cell lines, tumor immunity, and cancer progression ; 저용량 비스페놀A 노출이 유방암 세포의 전사체 발현, 종양면역 및 암 진행에 미치는 영향에 관한 연구 |
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| المؤلفون: | 김혜림 |
| المساهمون: | 문우경, Hyelim Kim, 의과대학 의과학과, 의과학전공 |
| بيانات النشر: | 서울대학교 대학원 |
| سنة النشر: | 2020 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | Bisphenol A, Breast cancer, Macrophage polarization, Transcriptional analysis, Tumor progression, 비스페놀A, 유방암, 대식세포 분극화, 전사체 분석, 암 진행, 610.72 |
| الوصف: | 학위논문 (박사) -- 서울대학교 대학원 : 의과대학 의과학과, 2020. 8. 문우경. ; Introduction: Bisphenol A (BPA) used in the manufacture of polycarbonate plastics and epoxy resin has structurally estrogen-like activity and recent studies have indicated that exposure to BPA in environment and daily life may account for the increased incidence of breast cancer in the industrialized world. Multiple in vivo and in vitro studies have reported that BPA exposure at low doses can result in breast neoplastic lesions. However, the correlation among BPA exposure, transcriptional alterations in breast cancer cells and breast cancer progression is not fully elucidated. In this study, we investigated whether chronic exposure to low-dose BPA affects transcriptome expression alterations and malignancy in different subtypes of breast cancer cells and mouse xenograft models. Methods: This study consists of two independent experiments. 1. ER-positive MCF-7, HER2-enriched SK-BR3, triple-negative MDA-MB-231 cells were used as breast cancer cell lines according to hormone receptor status. We analyzed the RNA-sequencing data from breast cancer cells treated with BPA at a low dose (10−8 M) for 30 days. Sequencing libraries were constructed using a QuantSeq 3mRNA-Seq Library Prep Kits. Differentially expressed genes (DEGs) were determined using BEDtools following mapping libraries using Bowtie2 software. Functional analysis was conducted by online public database DAVID, KEGG and PANTHER etc. Moreover, a functionally organized network of DEGs belonging to among MCF-7/BPA, SK-BR3/BPA and MDA-MB-231/BPA cells was created using the Cytoscape software platform. Afterward, we analyzed the correlation between BPA-exposed DEGs and survival rate of patients with various subtypes of breast cancer using public database BreastMark. 2. We investigated the effects of BPA (10−8 M) on the regulation of MCF10DCIS.com cells, ductal carcinoma in situ (DCIS), considered early-stage breast cancer and of RAW264.7, macrophages, the most abundant population in tumor immune ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| ردمك: | 978-0-00-000162-7 978-0-00-000000-2 0-00-000162-7 0-00-000000-0 |
| Relation: | 000000162776; https://hdl.handle.net/10371/170359; http://dcollection.snu.ac.kr/common/orgView/000000162776; I804:11032-000000162776; 000000000043▲000000000048▲000000162776▲ |
| الاتاحة: | https://hdl.handle.net/10371/170359 http://dcollection.snu.ac.kr/common/orgView/000000162776 |
| رقم الانضمام: | edsbas.FD44A65D |
| قاعدة البيانات: | BASE |
| ردمك: | 9780000001627 9780000000002 0000001627 0000000000 |
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