Academic Journal
Can urinary exosomes act as treatment response markers in prostate cancer?
| العنوان: | Can urinary exosomes act as treatment response markers in prostate cancer? |
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| المؤلفون: | Mitchell, Philip J., Welton, Joanne L., Staffurth, John Nicholas, Court, Jacquelyn, Mason, Malcolm David, Tabi, Zsuzsanna, Clayton, Aled |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2009 |
| المجموعة: | Cardiff University: ORCA (Online Research @ Cardiff) |
| مصطلحات موضوعية: | RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| الوصف: | Background Recently, nanometer sized vesicles (termed exosomes) have been described as a component of urine. Such vesicles may be a useful non-invasive source of markers in renal disease. Their utility as a source of markers in urological cancer remains unstudied. Our aim in this study was to investigate the feasibility and value of analysing urinary exosomes in prostate cancer patients undergoing standard therapy. Methods Ten patients (with locally advanced PCa) provided spot urine specimens at three time points during standard therapy. Patients received 3–6 months neoadjuvant androgen deprivation therapy prior to radical radiotherapy, comprising a single phase delivering 55 Gy in 20 fractions to the prostate and 44 Gy in 20 fractions to the pelvic nodes. Patients were continued on adjuvant ADT according to clinical need. Exosomes were purified, and the phenotype compared to exosomes isolated from the prostate cancer cell line LNcaP. A control group of 10 healthy donors was included. Serum PSA was used as a surrogate treatment response marker. Exosomes present in urine were quantified, and expression of prostate markers (PSA and PSMA) and tumour-associated marker 5T4 was examined. Results The quantity and quality of exosomes present in urine was highly variable, even though we handled all materials freshly and used methods optimized for obtaining highly pure exosomes. There was approx 2-fold decrease in urinary exosome content following 12 weeks ADT, but this was not sustained during radiotherapy. Nevertheless, PSA and PSMA were present in 20 of 24 PCa specimens, and not detected in healthy donor specimens. There was a clear treatment-related decrease in exosomal prostate markers in 1 (of 8) patient. Conclusion Evaluating urinary-exosomes remains difficult, given the variability of exosomes in urine specimens. Nevertheless, this approach holds promise as a non-invasive source of multiple markers of malignancy that could provide clinically useful information. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://orca.cardiff.ac.uk/id/eprint/31939/1/Mitchell%201479-5876-7-4.pdf; Mitchell, Philip J., Welton, Joanne L., Staffurth, John Nicholas https://orca.cardiff.ac.uk/view/cardiffauthors/A0702394.html orcid:0000-0002-7834-3172 orcid:0000-0002-7834-3172, Court, Jacquelyn, Mason, Malcolm David https://orca.cardiff.ac.uk/view/cardiffauthors/A008319Q.html orcid:0000-0003-1505-2869 orcid:0000-0003-1505-2869, Tabi, Zsuzsanna https://orca.cardiff.ac.uk/view/cardiffauthors/A047027C.html and Clayton, Aled https://orca.cardiff.ac.uk/view/cardiffauthors/A062480N.html orcid:0000-0002-3087-9226 orcid:0000-0002-3087-9226 2009. Can urinary exosomes act as treatment response markers in prostate cancer? Journal Of Translational Medicine 7 (4) , n/a. 10.1186/1479-5876-7-4 https://doi.org/10.1186/1479-5876-7-4 file https://orca.cardiff.ac.uk/id/eprint/31939/1/Mitchell%201479-5876-7-4.pdf |
| DOI: | 10.1186/1479-5876-7-4 |
| الاتاحة: | https://orca.cardiff.ac.uk/id/eprint/31939/ http://www.translational-medicine.com/content/7/1/4/ https://orca.cardiff.ac.uk/id/eprint/31939/1/Mitchell%201479-5876-7-4.pdf https://doi.org/10.1186/1479-5876-7-4 |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.F7468907 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/1479-5876-7-4 |
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