Report
Mechanical stress driven by rigidity sensing governs epithelial stability
| العنوان: | Mechanical stress driven by rigidity sensing governs epithelial stability |
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| المؤلفون: | Sonam, Surabhi, Balasubramaniam, Lakshmi, Lin, Shao-Zhen, Ivan, Ying Ming Yow, Jaumà, Irina Pi, Jebane, Cecile, Karnat, Marc, Toyama, Yusuke, Marcq, Philippe, Prost, Jacques, Mège, René-Marc, Rupprecht, Jean-François, Ladoux, Benoît |
| المساهمون: | Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre de Physique Théorique - UMR 7332 (CPT), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), CPT - E5 Physique statistique et systèmes complexes, Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Mechanobiology Institute Singapore (MBI), National University of Singapore (NUS), Laboratoire Physico-Chimie Curie Institut Curie (PCC), Institut Curie Paris -Institut de Chimie - CNRS Chimie (INC-CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université - Faculté de Physique (UFR 925), Sorbonne Université (SU) |
| المصدر: | https://hal.science/hal-03858265 ; 2022. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [PHYS]Physics [physics] |
| الوصف: | Epithelia act as a barrier against environmental stress and abrasion and i n vivo they are continuously exposed to environments of various mechanical properties. The impact of this environment on epithelial integrity remains elusive. By culturing epithelial cells on 2D hydrogels, we observe a loss of epithelial monolayer integrity through spontaneous hole formation when grown on soft substrates. Substrate stiffness triggers an unanticipated mechanical switch of epithelial monolayers from tensile on soft to compressive on stiff substrates. Through active nematic modelling, we find unique patterns of cell shape texture called nematic topological defects that underpin large isotropic stress fluctuations at certain locations thereby triggering mechanical failure of the monolayer and hole opening. Our results show that substrate stiffness provides feedback on monolayer mechanical state and that topological defects can trigger stochastic mechanical failure, with potential application towards a mechanistic understanding of compromised epithelial integrity in bacterial infection, tumor progression and morphogenesis. |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | hal-03858265; https://hal.science/hal-03858265; BIORXIV: 2022.03.10.483785 |
| DOI: | 10.1101/2022.03.10.483785 |
| الاتاحة: | https://hal.science/hal-03858265 https://doi.org/10.1101/2022.03.10.483785 |
| رقم الانضمام: | edsbas.F13DE07E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2022.03.10.483785 |
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