Report
Mitochondrial DNA heteroplasmy distinguishes disease manifestation in PINK1- and PRKN-linked Parkinson's disease 2022.05.17.22275087
| العنوان: | Mitochondrial DNA heteroplasmy distinguishes disease manifestation in PINK1- and PRKN-linked Parkinson's disease 2022.05.17.22275087 |
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| المؤلفون: | Trinh, Joanne, Hicks, Andrew A., Koenig, Inke R., Delcambre, Sylvie, Lueth, Theresa, Schaake, Susen, Wasner, Kobi, Ghelfi, Jenny, Borsche, Max, Vilarino-Guell, Carles, Hentati, Faycel, Germer, Elisabeth L., Bauer, Peter, Takanashi, Masashi, Kostic, Vladimir, Lang, Anthony E., Brueggeman, Norbert, Pramstaller, Peter P., Pichler, Irene, Rajput, Alex, Hattori, Nobutaka, Farrer, Matthew J., Lohmann, Katja, May, Patrick, Weissensteiner, Hansi, Klein, Christine, Grünewald, Anne |
| المساهمون: | Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group), Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (A. Grünewald Group) |
| بيانات النشر: | Cold Spring Harbor Laboratory Press |
| سنة النشر: | 2022 |
| المجموعة: | University of Luxembourg: ORBilu - Open Repository and Bibliography |
| مصطلحات موضوعية: | MtDNA, heteroplasmy, Parkinson's disease, PINK1, PRKN, Life sciences, Genetics & genetic processes, Human health sciences, Neurology, Sciences du vivant, Génétique & processus génétiques, Sciences de la santé humaine, Neurologie |
| الوصف: | Biallelic mutations in PINK1 and PRKN cause recessively inherited Parkinson's disease (PD). Though some studies suggest that PINK1/PRKN monoallelic mutations may not contribute to risk, deep phenotyping assessment showed that PINK1 or PRKN monoallelic pathogenic variants were at a significantly higher rate in PD compared to controls. Given the established role of PINK1 and Parkin in regulating mitochondrial dynamics, we explored mitochondrial DNA (mtDNA) integrity and inflammation as potential disease modifiers in carriers of mutations in these genes. MtDNA integrity, global gene expression and serum cytokine levels were investigated in a large collection of biallelic (n=84) and monoallelic (n=170) carriers of PINK1/PRKN mutations, iPD patients (n=67) and controls (n=90). Affected and unaffected PINK1/PRKN monoallelic mutation carriers can be distinguished by heteroplasmic mtDNA variant load (AUC=0.83, CI:0.74-0.93). Biallelic PINK1/PRKN mutation carriers harbor more heteroplasmic mtDNA variants in blood (p=0.0006, Z=3.63) compared to monoallelic mutation carriers. This enrichment was confirmed in iPSC-derived and postmortem midbrain neurons from biallelic PRKN-PD patients. Lastly, the heteroplasmic mtDNA variant load was found to correlate with IL6 levels in PINK1/PRKN mutation carriers (r=0.57, p=0.0074). PINK1/PRKN mutations predispose individuals to mtDNA variant accumulation in a dose- and disease-dependent manner. MtDNA variant load over time is a potential marker of disease manifestation in PINK1/PRKN mutation carriers.Competing Interest StatementThe authors have declared no competing interest.Funding StatementThe authors wish to thank the many patients and their families who volunteered, and the efforts of the many clinical teams involved. Funding has been obtained from the German Research Foundation (ProtectMove; FOR 2488, GR 3731/5-1; SE 2608/2-1; KO 2250/7-1), the Luxembourg National Research Fund in the ATTRACT (Model-IPD, FNR9631103), NCER-PD (FNR11264123) and INTER programmes (ProtectMove, ... |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | https://www.medrxiv.org/content/early/2022/05/19/2022.05.17.22275087; FNR14429377 - Reduced Penetrance In Hereditary Movement Disorders: Elucidating Mechanisms Of Endogenous Disease Protection, 2020 (01/07/2020-30/06/2023) - Anne Grünewald; https://orbilu.uni.lu/handle/10993/51280; info:hdl:10993/51280; https://orbilu.uni.lu/bitstream/10993/51280/1/2022.05.17.22275087v1.full.pdf |
| DOI: | 10.1101/2022.05.17.22275087 |
| الاتاحة: | https://orbilu.uni.lu/handle/10993/51280 https://orbilu.uni.lu/bitstream/10993/51280/1/2022.05.17.22275087v1.full.pdf https://doi.org/10.1101/2022.05.17.22275087 |
| Rights: | open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.EA42D026 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2022.05.17.22275087 |
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