Academic Journal
Inclusion body formation by interleukin‐1β depends on the thermal sensitivity of a folding intermediate
| العنوان: | Inclusion body formation by interleukin‐1β depends on the thermal sensitivity of a folding intermediate |
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| المؤلفون: | Wetzel, Ronald, Chrunyk, Boris A |
| المصدر: | FEBS Letters ; volume 350, issue 2-3, page 245-248 ; ISSN 0014-5793 1873-3468 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1994 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | We show that sequence and growth temperature effects on IB formation in the small, monomeric β‐barrel protein interleukin‐1β (IL‐1β) can be quantitatively reproduced in an in vitro system in which IL‐1β is refolded from denaturant at different temperatures. The results suggest that temperature and mutational effects on IB formation may be based on intrinsic properties of the protein sequence rather than interactions with chaperones or other cellular factors. We also report striking correlations of IB formation with mutation‐dependent changes in residue hydrophobicity. The nature of these trends differs considerably with residue position, however, suggesting that they are mediated by particular local environments created by an ordered structure. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/0014-5793(94)00775-6 |
| DOI: | 10.1016/0014-5793%2894%2900775-6 |
| الاتاحة: | http://dx.doi.org/10.1016/0014-5793(94)00775-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2894%2900775-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(94)00775-6 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2894%2900775-6 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.E5FF7B25 |
| قاعدة البيانات: | BASE |
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