Academic Journal
PIWI silencing mechanism involving the retrotransposon nimbus orchestrates resistance to infection with Schistosoma mansoni in the snail vector, Biomphalaria glabrata.
| العنوان: | PIWI silencing mechanism involving the retrotransposon nimbus orchestrates resistance to infection with Schistosoma mansoni in the snail vector, Biomphalaria glabrata. |
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| المؤلفون: | Michael Smith, Swara Yadav, Olayemi G Fagunloye, Nana Adjoa Pels, Daniel A Horton, Nashwah Alsultan, Andrea Borns, Carolyn Cousin, Freddie Dixon, Victoria H Mann, Clarence Lee, Paul J Brindley, Najib M El-Sayed, Joanna M Bridger, Matty Knight |
| المصدر: | PLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009094 (2021) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
| الوصف: | Background Schistosomiasis remains widespread in many regions despite efforts at its elimination. By examining changes in the transcriptome at the host-pathogen interface in the snail Biomphalaria glabrata and the blood fluke Schistosoma mansoni, we previously demonstrated that an early stress response in juvenile snails, manifested by induction of heat shock protein 70 (Hsp 70) and Hsp 90 and of the reverse transcriptase (RT) domain of the B. glabrata non-LTR- retrotransposon, nimbus, were critical for B. glabrata susceptibility to S. mansoni. Subsequently, juvenile B. glabrata BS-90 snails, resistant to S. mansoni at 25°C become susceptible by the F2 generation when maintained at 32°C, indicating an epigenetic response. Methodology/principal findings To better understand this plasticity in susceptibility of the BS-90 snail, mRNA sequences were examined from S. mansoni exposed juvenile BS-90 snails cultured either at 25°C (non-permissive temperature) or 32°C (permissive). Comparative analysis of transcriptomes from snails cultured at the non-permissive and permissive temperatures revealed that whereas stress related transcripts dominated the transcriptome of susceptible BS-90 juvenile snails at 32°C, transcripts encoding proteins with a role in epigenetics, such as PIWI (BgPiwi), chromobox protein homolog 1 (BgCBx1), histone acetyltransferase (BgHAT), histone deacetylase (BgHDAC) and metallotransferase (BgMT) were highly expressed in those cultured at 25°C. To identify robust candidate transcripts that will underscore the anti-schistosome phenotype in B. glabrata, further validation of the differential expression of the above transcripts was performed by using the resistant BS-90 (25°C) and the BBO2 susceptible snail stock whose genome has now been sequenced and represents an invaluable resource for molecular studies in B. glabrata. A role for BgPiwi in B. glabrata susceptibility to S. mansoni, was further examined by using siRNA corresponding to the BgPiwi encoding transcript to suppress expression of BgPiwi, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1935-2727 1935-2735 |
| Relation: | https://doi.org/10.1371/journal.pntd.0009094; https://doaj.org/toc/1935-2727; https://doaj.org/toc/1935-2735; https://doaj.org/article/347e2f90ad1d4bc2b77c6bda5239d9af |
| DOI: | 10.1371/journal.pntd.0009094 |
| الاتاحة: | https://doi.org/10.1371/journal.pntd.0009094 https://doaj.org/article/347e2f90ad1d4bc2b77c6bda5239d9af |
| رقم الانضمام: | edsbas.DDD6BB21 |
| قاعدة البيانات: | BASE |
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