Academic Journal
أعرض في EDS

Cytotoxic Activity of Non-specific Lipid Transfer Protein (nsLTP1) from Ajwain (Trachyspermum ammi) Seeds

التفاصيل البيبلوغرافية
العنوان: Cytotoxic Activity of Non-specific Lipid Transfer Protein (nsLTP1) from Ajwain (Trachyspermum ammi) Seeds
المؤلفون: Alshammari, Saud O., Aldakhil, Taibah, Alshammari, Qamar A., Salehi, David, Ahmed, Aftab
المصدر: Pharmacy Faculty Articles and Research
بيانات النشر: Chapman University Digital Commons
سنة النشر: 2022
المجموعة: Chapman University Digital Commons
مصطلحات موضوعية: Ajwain seeds, Protein, nsLTP, Anticancer activity, Apoptosis, Serum stability, CD spectroscopy, Flow cytometry, Amino Acids, Peptides, and Proteins, Cancer Biology, Food Chemistry, Medicinal and Pharmaceutical Chemistry, Other Plant Sciences, Therapeutics
الوصف: Background Trachyspermum ammi, commonly known as Ajwain, is a member of the Apiaceae family. It is a therapeutic herbal spice with diverse pharmacological properties, used in traditional medicine for various ailments. However, all previous studies were conducted using small molecule extracts, leaving the protein’s bioactivity undiscovered. Aim The current study aimed to demonstrate the cytotoxic activity of Ajwain non-specific lipid transfer protein (nsLTP1) in normal breast (MCF10A), breast cancer (MCF-7), and pancreatic cancer (AsPC-1) cell lines. Also, to evaluate its structural stability in human serum as well as at high temperature conditions. Methods The cytotoxic activity of Ajwain nsLTP1 was evaluated in MCF-7 and AsPC-1 cell lines using MTT assay. Annexin V-FITC and PI staining were used to detect the early apoptotic and late apoptotic cells. The role of nsLTP1 in inducing apoptosis was further studied by quantifying Bcl-2, Bax, Caspase-3, Survivin, EGFR, and VEGF genes expression using RT-PCR. CD spectroscopy analyzed the nsLTP1 conformational changes after thermal treatment for structure stability determination. The RP-HPLC was used to analyze the nsLTP1 degradation rate in human serum at different time intervals incubated at 37 °C. Results Ajwain nsLTP1 showed a potent cytotoxic effect in MCF-7 and AsPC-1. The IC50 value obtained in MCF-7 was 8.21 μM, while for AsPC-1 4.17 μM. The effect of nsLTP1 on stimulating apoptosis revealed that the proportions of apoptotic cells in both cell lines were relatively increased depending on the concentration. The apoptotic cells percentage at 20 μM was in MCF-7 71% (***P < 0.001) and AsPC-1 88% (***P < 0.001). These results indicate that nsLTP1 might efficaciously induce apoptosis in multiple types of cancerous cells. Genes expression in MCF-7 and AsPC-1 showed significant upregulation in Bax and Caspase-3 and downregulation in Bcl-2, Survivin, EGFR, and VEGF protein. The CD analysis of nsLTP1 showed a significant thermostable property. In serum, nsLTP1 ...
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: unknown
Relation: https://digitalcommons.chapman.edu/pharmacy_articles/908; https://digitalcommons.chapman.edu/cgi/viewcontent.cgi?article=1909&context=pharmacy_articles
الاتاحة: https://digitalcommons.chapman.edu/pharmacy_articles/908
https://digitalcommons.chapman.edu/cgi/viewcontent.cgi?article=1909&context=pharmacy_articles
Rights: The authors ; http://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.DBA9DE65
قاعدة البيانات: BASE
ResultId 1
Header edsbas
BASE
edsbas.DBA9DE65
938
3
Academic Journal
academicJournal
937.599182128906
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.DBA9DE65&custid=s6537998&authtype=sso
FullText Array ( [Availability] => 0 )
Array ( [0] => Array ( [Url] => https://digitalcommons.chapman.edu/pharmacy_articles/908# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) )
Items Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Cytotoxic Activity of Non-specific Lipid Transfer Protein (nsLTP1) from Ajwain (Trachyspermum ammi) Seeds )
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Alshammari%2C+Saud+O%2E%22&quot;&gt;Alshammari, Saud O.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Aldakhil%2C+Taibah%22&quot;&gt;Aldakhil, Taibah&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Alshammari%2C+Qamar+A%2E%22&quot;&gt;Alshammari, Qamar A.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Salehi%2C+David%22&quot;&gt;Salehi, David&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Ahmed%2C+Aftab%22&quot;&gt;Ahmed, Aftab&lt;/searchLink&gt; )
Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Pharmacy Faculty Articles and Research )
Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Chapman University Digital Commons )
Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2022 )
Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Chapman University Digital Commons )
Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Ajwain+seeds%22&quot;&gt;Ajwain seeds&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Protein%22&quot;&gt;Protein&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22nsLTP%22&quot;&gt;nsLTP&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Anticancer+activity%22&quot;&gt;Anticancer activity&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Apoptosis%22&quot;&gt;Apoptosis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Serum+stability%22&quot;&gt;Serum stability&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22CD+spectroscopy%22&quot;&gt;CD spectroscopy&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Flow+cytometry%22&quot;&gt;Flow cytometry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Amino+Acids%22&quot;&gt;Amino Acids&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Peptides%22&quot;&gt;Peptides&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22and+Proteins%22&quot;&gt;and Proteins&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cancer+Biology%22&quot;&gt;Cancer Biology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Food+Chemistry%22&quot;&gt;Food Chemistry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Medicinal+and+Pharmaceutical+Chemistry%22&quot;&gt;Medicinal and Pharmaceutical Chemistry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Other+Plant+Sciences%22&quot;&gt;Other Plant Sciences&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Therapeutics%22&quot;&gt;Therapeutics&lt;/searchLink&gt; )
Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Background Trachyspermum ammi, commonly known as Ajwain, is a member of the Apiaceae family. It is a therapeutic herbal spice with diverse pharmacological properties, used in traditional medicine for various ailments. However, all previous studies were conducted using small molecule extracts, leaving the protein’s bioactivity undiscovered. Aim The current study aimed to demonstrate the cytotoxic activity of Ajwain non-specific lipid transfer protein (nsLTP1) in normal breast (MCF10A), breast cancer (MCF-7), and pancreatic cancer (AsPC-1) cell lines. Also, to evaluate its structural stability in human serum as well as at high temperature conditions. Methods The cytotoxic activity of Ajwain nsLTP1 was evaluated in MCF-7 and AsPC-1 cell lines using MTT assay. Annexin V-FITC and PI staining were used to detect the early apoptotic and late apoptotic cells. The role of nsLTP1 in inducing apoptosis was further studied by quantifying Bcl-2, Bax, Caspase-3, Survivin, EGFR, and VEGF genes expression using RT-PCR. CD spectroscopy analyzed the nsLTP1 conformational changes after thermal treatment for structure stability determination. The RP-HPLC was used to analyze the nsLTP1 degradation rate in human serum at different time intervals incubated at 37 &#176;C. Results Ajwain nsLTP1 showed a potent cytotoxic effect in MCF-7 and AsPC-1. The IC50 value obtained in MCF-7 was 8.21 μM, while for AsPC-1 4.17 μM. The effect of nsLTP1 on stimulating apoptosis revealed that the proportions of apoptotic cells in both cell lines were relatively increased depending on the concentration. The apoptotic cells percentage at 20 μM was in MCF-7 71% (***P &lt; 0.001) and AsPC-1 88% (***P &lt; 0.001). These results indicate that nsLTP1 might efficaciously induce apoptosis in multiple types of cancerous cells. Genes expression in MCF-7 and AsPC-1 showed significant upregulation in Bax and Caspase-3 and downregulation in Bcl-2, Survivin, EGFR, and VEGF protein. The CD analysis of nsLTP1 showed a significant thermostable property. In serum, nsLTP1 ... )
Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => text )
Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => application/pdf )
Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => unknown )
Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://digitalcommons.chapman.edu/pharmacy_articles/908; https://digitalcommons.chapman.edu/cgi/viewcontent.cgi?article=1909&amp;context=pharmacy_articles )
Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://digitalcommons.chapman.edu/pharmacy_articles/908&lt;br /&gt;https://digitalcommons.chapman.edu/cgi/viewcontent.cgi?article=1909&amp;context=pharmacy_articles )
Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => The authors ; http://creativecommons.org/licenses/by/4.0/ )
Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.DBA9DE65 )
RecordInfo Array ( [BibEntity] => Array ( [Languages] => Array ( [0] => Array ( [Text] => unknown ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => Ajwain seeds [Type] => general ) [1] => Array ( [SubjectFull] => Protein [Type] => general ) [2] => Array ( [SubjectFull] => nsLTP [Type] => general ) [3] => Array ( [SubjectFull] => Anticancer activity [Type] => general ) [4] => Array ( [SubjectFull] => Apoptosis [Type] => general ) [5] => Array ( [SubjectFull] => Serum stability [Type] => general ) [6] => Array ( [SubjectFull] => CD spectroscopy [Type] => general ) [7] => Array ( [SubjectFull] => Flow cytometry [Type] => general ) [8] => Array ( [SubjectFull] => Amino Acids [Type] => general ) [9] => Array ( [SubjectFull] => Peptides [Type] => general ) [10] => Array ( [SubjectFull] => and Proteins [Type] => general ) [11] => Array ( [SubjectFull] => Cancer Biology [Type] => general ) [12] => Array ( [SubjectFull] => Food Chemistry [Type] => general ) [13] => Array ( [SubjectFull] => Medicinal and Pharmaceutical Chemistry [Type] => general ) [14] => Array ( [SubjectFull] => Other Plant Sciences [Type] => general ) [15] => Array ( [SubjectFull] => Therapeutics [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Cytotoxic Activity of Non-specific Lipid Transfer Protein (nsLTP1) from Ajwain (Trachyspermum ammi) Seeds [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Alshammari, Saud O. ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Aldakhil, Taibah ) ) ) [2] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Alshammari, Qamar A. ) ) ) [3] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Salehi, David ) ) ) [4] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Ahmed, Aftab ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 01 [M] => 01 [Type] => published [Y] => 2022 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-locals [Value] => edsbas ) [1] => Array ( [Type] => issn-locals [Value] => edsbas.oa ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Pharmacy Faculty Articles and Research [Type] => main ) ) ) ) ) ) )
IllustrationInfo