Academic Journal
Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles
| العنوان: | Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles |
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| المؤلفون: | Leroy, Amandine, Devaux, M.F., Fanuel, Mathieu, Chauvet, Hugo, Durand, Sylvie, Alvarado, Camille, Habrant, Anouck, Sandt, Christophe, Rogniaux, Hélène, Paës, Gabriel, Guillon, Fabienne |
| المساهمون: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INRAE, PROBE Research Infrastructure, Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), DISCO beamline, Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0960-8524 ; Bioresource Technology ; https://hal.inrae.fr/hal-03664146 ; Bioresource Technology, 2022, 353, pp.1-10. ⟨10.1016/j.biortech.2022.127140⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) |
| مصطلحات موضوعية: | Lignocellulose, Hot water pretreatment, Microspectrometry, Fluorescence, FTIR microspectroscopy, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [CHIM.ANAL]Chemical Sciences/Analytical chemistry |
| الوصف: | International audience ; This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale. Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation. Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells. This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-802578-6 0-00-802578-9 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35405211; PUBMED: 35405211; WOS: 000802578900004 |
| DOI: | 10.1016/j.biortech.2022.127140 |
| الاتاحة: | https://hal.inrae.fr/hal-03664146 https://hal.inrae.fr/hal-03664146v1/document https://hal.inrae.fr/hal-03664146v1/file/S0960852422004692.pdf https://doi.org/10.1016/j.biortech.2022.127140 |
| Rights: | http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.D94D381E |
| قاعدة البيانات: | BASE |
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