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Comparative Evaluation of PCR and Imprint Smear Microscopy Analyses of Skin Biopsy Specimens in Diagnosis of Macular, Papular, and Mixed Papulo-Nodular Lesions of Post-Kala-Azar Dermal Leishmaniasis

التفاصيل البيبلوغرافية
العنوان: Comparative Evaluation of PCR and Imprint Smear Microscopy Analyses of Skin Biopsy Specimens in Diagnosis of Macular, Papular, and Mixed Papulo-Nodular Lesions of Post-Kala-Azar Dermal Leishmaniasis
المؤلفون: Verma, Neena, Singh, Dharmendra, Pandey, Krishna, Das, Vidya Nand Rabi, Lal, Chandra Shekhar, Verma, Rakesh Bihari, Sinha, Prabhat Kumar, Das, Pradeep
المصدر: Journal of Clinical Microbiology ; volume 51, issue 12, page 4217-4219 ; ISSN 0095-1137 1098-660X
بيانات النشر: American Society for Microbiology
سنة النشر: 2013
الوصف: Diagnosis of post-kala-azar dermal leishmaniasis (PKDL), particularly the macular form, is difficult when based on microscopy. This study compared the results of nested PCR (91.9% positive samples) with imprint smear microscopy (70.9% positive samples) for 62 PKDL samples. We found that nested PCR, which indicated 87.5% positivity for the macular lesions, compared to 41.6% positivity by imprint smear microscopy, is an efficient method for early diagnosis of PKDL.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1128/jcm.01482-13
DOI: 10.1128/JCM.01482-13
الاتاحة: http://dx.doi.org/10.1128/jcm.01482-13
https://journals.asm.org/doi/pdf/10.1128/JCM.01482-13
Rights: https://journals.asm.org/non-commercial-tdm-license
رقم الانضمام: edsbas.D903A00F
قاعدة البيانات: BASE