Academic Journal
Response to anti-VEGF-A treatment of retinal pigment epithelial cells in vitro.
| العنوان: | Response to anti-VEGF-A treatment of retinal pigment epithelial cells in vitro. |
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| المؤلفون: | PUDDU, ALESSANDRA, SANGUINETI, ROBERTA, TRAVERSO, CARLO, VIVIANI, GIORGIO LUCIANO, NICOLO', MASSIMO |
| المساهمون: | Puddu, Alessandra, Sanguineti, Roberta, Traverso, Carlo, Viviani, GIORGIO LUCIANO, Nicolo', Massimo |
| بيانات النشر: | Wichtig DEU |
| سنة النشر: | 2016 |
| المجموعة: | Università degli Studi di Genova: CINECA IRIS |
| الوصف: | PURPOSE: The neovascular or wet form of age-related macular degeneration is characterized by the growth of abnormal blood vessels in the retina stimulated by vascular endothelial growth factors (VEGF). In the last decade, several anti-VEGF drugs have been developed for treating neovascular diseases of the eyes. This study was conducted to compare the effects of 2 anti-VEGF-A drugs, ranibizumab and aflibercept, on the expression and secretion of VEGF family members in retinal pigment epithelial cells (RPE) in vitro. METHODS: ARPE-19 cells were exposed for 24 hours to ranibizumab or aflibercept at clinical dose concentration. Cell viability and expression and secretion of VEGF-A, VEGF-B, VEGF-C, and placental growth factor (PlGF) were evaluated respectively by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. RESULTS: Ranibizumab and aflibercept did not affect ARPE-19 cell viability after 24 hours of treatment. Ranibizumab increased expression of VEGF-A and PlGF. On the contrary, expression and secretion of VEGF-C was decreased by ranibizumab. PlGF secretion was not affected by ranibizumab. Aflibercept strongly increased VEGF-A and PlGF expression but reduced their detection on the culture media, and decreased expression and secretion of VEGF-C. No effect on expression and secretion of VEGF-B was observed after exposure to these drugs. CONCLUSIONS: Ranibizumab and aflibercept exert similar effects on VEGF expression and secretion, leading to establishing an antiangiogenic environment. Increased VEGF-A expression observed in RPE cells treated with these drugs suggests a compensatory response of the cells to the lack of VEGF-A. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000393688500019; volume:Aug 4;26(5):425-30; firstpage:425; lastpage:430; numberofpages:6; journal:EUROPEAN JOURNAL OF OPHTHALMOLOGY; http://hdl.handle.net/11567/842610; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84984911050 |
| DOI: | 10.5301/ejo.5000786 |
| الاتاحة: | http://hdl.handle.net/11567/842610 https://doi.org/10.5301/ejo.5000786 |
| رقم الانضمام: | edsbas.D6B7D270 |
| قاعدة البيانات: | BASE |
| DOI: | 10.5301/ejo.5000786 |
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