Academic Journal
FMNL1 and mDia1 promote efficient T cell migration through complex environments via distinct mechanisms
| العنوان: | FMNL1 and mDia1 promote efficient T cell migration through complex environments via distinct mechanisms |
|---|---|
| المؤلفون: | Ashton L. Sigler, Scott B. Thompson, Logan Ellwood-Digel, Adithan Kandasamy, Mary J. Michaels, Dean Thumkeo, Shuh Narumiya, Juan C. Del Alamo, Jordan Jacobelli |
| المصدر: | Frontiers in Immunology, Vol 15 (2024) |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | T cell, formins, cell migration, motility, FMNL1, mDia1, Immunologic diseases. Allergy, RC581-607 |
| الوصف: | Lymphocyte trafficking and migration through tissues is critical for adaptive immune function and, to perform their roles, T cells must be able to navigate through diverse tissue environments that present a range of mechanical challenges. T cells predominantly express two members of the formin family of actin effectors, Formin-like 1 (FMNL1) and mammalian diaphanous-related formin 1 (mDia1). While both FMNL1 and mDia1 have been studied individually, they have not been directly compared to determine functional differences in promoting T cell migration. Through in vivo analysis and the use of in vitro 2D and 3D model environments, we demonstrate that FMNL1 and mDia1 are both required for effective T cell migration, but they have different localization and roles in T cells, with specific environment-dependent functions. We found that mDia1 promotes general motility in 3D environments in conjunction with Myosin-II activity. We also show that, while mDia1 is almost entirely in the cytoplasmic compartment, a portion of FMNL1 physically associates with the nucleus. Furthermore, FMNL1 localizes to the rear of migrating T cells and contributes to efficient migration by promoting deformation of the rigid T cell nucleus in confined environments. Overall, our data indicates that while FMNL1 and mDia1 have similar mechanisms of actin polymerization, they have distinct roles in promoting T cell migration. This suggests that differential modulation of FMNL1 and mDia1 can be an attractive therapeutic route to fine-tune T cell migration behavior. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1467415/full; https://doaj.org/toc/1664-3224; https://doaj.org/article/8d8a242143d24c529f8431db63d56272 |
| DOI: | 10.3389/fimmu.2024.1467415 |
| الاتاحة: | https://doi.org/10.3389/fimmu.2024.1467415 https://doaj.org/article/8d8a242143d24c529f8431db63d56272 |
| رقم الانضمام: | edsbas.D65C1BA0 |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.D65C1BA0 995 3 Academic Journal academicJournal 995.377258300781 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.D65C1BA0&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doi.org/10.3389/fimmu.2024.1467415# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => FMNL1 and mDia1 promote efficient T cell migration through complex environments via distinct mechanisms
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Ashton+L%2E+Sigler%22">Ashton L. Sigler</searchLink><br /><searchLink fieldCode="AR" term="%22Scott+B%2E+Thompson%22">Scott B. Thompson</searchLink><br /><searchLink fieldCode="AR" term="%22Logan+Ellwood-Digel%22">Logan Ellwood-Digel</searchLink><br /><searchLink fieldCode="AR" term="%22Adithan+Kandasamy%22">Adithan Kandasamy</searchLink><br /><searchLink fieldCode="AR" term="%22Mary+J%2E+Michaels%22">Mary J. Michaels</searchLink><br /><searchLink fieldCode="AR" term="%22Dean+Thumkeo%22">Dean Thumkeo</searchLink><br /><searchLink fieldCode="AR" term="%22Shuh+Narumiya%22">Shuh Narumiya</searchLink><br /><searchLink fieldCode="AR" term="%22Juan+C%2E+Del+Alamo%22">Juan C. Del Alamo</searchLink><br /><searchLink fieldCode="AR" term="%22Jordan+Jacobelli%22">Jordan Jacobelli</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Frontiers in Immunology, Vol 15 (2024) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Frontiers Media S.A. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2024 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Directory of Open Access Journals: DOAJ Articles ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22T+cell%22">T cell</searchLink><br /><searchLink fieldCode="DE" term="%22formins%22">formins</searchLink><br /><searchLink fieldCode="DE" term="%22cell+migration%22">cell migration</searchLink><br /><searchLink fieldCode="DE" term="%22motility%22">motility</searchLink><br /><searchLink fieldCode="DE" term="%22FMNL1%22">FMNL1</searchLink><br /><searchLink fieldCode="DE" term="%22mDia1%22">mDia1</searchLink><br /><searchLink fieldCode="DE" term="%22Immunologic+diseases%2E+Allergy%22">Immunologic diseases. Allergy</searchLink><br /><searchLink fieldCode="DE" term="%22RC581-607%22">RC581-607</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Lymphocyte trafficking and migration through tissues is critical for adaptive immune function and, to perform their roles, T cells must be able to navigate through diverse tissue environments that present a range of mechanical challenges. T cells predominantly express two members of the formin family of actin effectors, Formin-like 1 (FMNL1) and mammalian diaphanous-related formin 1 (mDia1). While both FMNL1 and mDia1 have been studied individually, they have not been directly compared to determine functional differences in promoting T cell migration. Through in vivo analysis and the use of in vitro 2D and 3D model environments, we demonstrate that FMNL1 and mDia1 are both required for effective T cell migration, but they have different localization and roles in T cells, with specific environment-dependent functions. We found that mDia1 promotes general motility in 3D environments in conjunction with Myosin-II activity. We also show that, while mDia1 is almost entirely in the cytoplasmic compartment, a portion of FMNL1 physically associates with the nucleus. Furthermore, FMNL1 localizes to the rear of migrating T cells and contributes to efficient migration by promoting deformation of the rigid T cell nucleus in confined environments. Overall, our data indicates that while FMNL1 and mDia1 have similar mechanisms of actin polymerization, they have distinct roles in promoting T cell migration. This suggests that differential modulation of FMNL1 and mDia1 can be an attractive therapeutic route to fine-tune T cell migration behavior. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://www.frontiersin.org/articles/10.3389/fimmu.2024.1467415/full; https://doaj.org/toc/1664-3224; https://doaj.org/article/8d8a242143d24c529f8431db63d56272 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.3389/fimmu.2024.1467415 ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://doi.org/10.3389/fimmu.2024.1467415<br />https://doaj.org/article/8d8a242143d24c529f8431db63d56272 ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.D65C1BA0 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3389/fimmu.2024.1467415
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => T cell
[Type] => general
)
[1] => Array
(
[SubjectFull] => formins
[Type] => general
)
[2] => Array
(
[SubjectFull] => cell migration
[Type] => general
)
[3] => Array
(
[SubjectFull] => motility
[Type] => general
)
[4] => Array
(
[SubjectFull] => FMNL1
[Type] => general
)
[5] => Array
(
[SubjectFull] => mDia1
[Type] => general
)
[6] => Array
(
[SubjectFull] => Immunologic diseases. Allergy
[Type] => general
)
[7] => Array
(
[SubjectFull] => RC581-607
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => FMNL1 and mDia1 promote efficient T cell migration through complex environments via distinct mechanisms
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ashton L. Sigler
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Scott B. Thompson
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Logan Ellwood-Digel
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Adithan Kandasamy
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Mary J. Michaels
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Dean Thumkeo
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Shuh Narumiya
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Juan C. Del Alamo
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jordan Jacobelli
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2024
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsbas.oa
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Frontiers in Immunology, Vol 15 (2024
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |