Academic Journal
An Alveolata secretory machinery adapted to parasite host cell invasion
| العنوان: | An Alveolata secretory machinery adapted to parasite host cell invasion |
|---|---|
| المؤلفون: | Aquilini, Eleonora, Mendonça Cova, Marta, Kumar Mageswaran, Shrawan, dos Santos Pacheco, Nicolas, Sparvoli, Daniela, Penarete-Vargas, Diana, Najm, Rania, Graindorge, Arnault, Suarez, Catherine, Maynadier, Marjorie, Berry-Sterkers, Laurence, Urbach, Serge, Fahy, Pilar, Guérin, Amandine, Striepen, Boris, Dubremetz, Jean-François, Chang, Yi-Wei, Turkewitz, Aaron, Lebrun, Maryse |
| المساهمون: | Laboratory of Pathogen Host Interactions Montpellier (LPHI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), University of Pennsylvania, University of Chicago, Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Genève = University of Geneva (UNIGE), ANR-16-CE15-0010,JUNCTIONPARTNERS,Caractérisation des partenaires de la cellule hôte impliqués dans la formation de la jonction mobile chez les Apicomplexes(2016) |
| المصدر: | ISSN: 2058-5276. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2021 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | MESH: Alveolata, MESH: Cell Membrane, MESH: Exocytosis, MESH: Host-Parasite Interactions, MESH: Humans, MESH: Organelles, MESH: Protozoan Proteins, MESH: Secretory Vesicles, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Apicomplexa are unicellular eukaryotes and obligate intracellular parasites, including Plasmodium (the causative agent of malaria) and Toxoplasma (one of the most widespread zoonotic pathogens). Rhoptries, one of their specialized secretory organelles, undergo regulated exocytosis during invasion1. Rhoptry proteins are injected directly into the host cell to support invasion and subversion of host immune function2. The mechanism by which they are discharged is unclear and appears distinct from those in bacteria, yeast, animals and plants. Here, we show that rhoptry secretion in Apicomplexa shares structural and genetic elements with the exocytic machinery of ciliates, their free-living relatives. Rhoptry exocytosis depends on intramembranous particles in the shape of a rosette embedded into the plasma membrane of the parasite apex. Formation of this rosette requires multiple non-discharge (Nd) proteins conserved and restricted to Ciliata, Dinoflagellata and Apicomplexa that together constitute the superphylum Alveolata. We identified Nd6 at the site of exocytosis in association with an apical vesicle. Sandwiched between the rosette and the tip of the rhoptry, this vesicle appears as a central element of the rhoptry secretion machine. Our results describe a conserved secretion system that was adapted to provide defence for free-living unicellular eukaryotes and host cell injection in intracellular parasites. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/33495622; inserm-03654158; https://inserm.hal.science/inserm-03654158; https://inserm.hal.science/inserm-03654158/document; https://inserm.hal.science/inserm-03654158/file/Aquilini%20%26%20Cova%20et%20al.%20Main%20Text%20and%20Figures.pdf; PUBMED: 33495622; PUBMEDCENTRAL: PMC8886610 |
| DOI: | 10.1038/s41564-020-00854-z |
| الاتاحة: | https://inserm.hal.science/inserm-03654158 https://inserm.hal.science/inserm-03654158/document https://inserm.hal.science/inserm-03654158/file/Aquilini%20%26%20Cova%20et%20al.%20Main%20Text%20and%20Figures.pdf https://doi.org/10.1038/s41564-020-00854-z |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.D2FD24D1 |
| قاعدة البيانات: | BASE |
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