التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of loopamp assay for the diagnosis of pulmonary tuberculosis in Cambodia |
| المؤلفون: |
Cheng, S., Pheng, S. H., Seiha, H., Marks, G. B., /Banuls, Anne-Laure, Mao, T. E., Kerleguer, A. |
| سنة النشر: |
2020 |
| المجموعة: |
IRD (Institute de recherche pour le développement): Horizon |
| جغرافية الموضوع: |
CAMBODGE |
| الوصف: |
The Loopamp (TM) MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate. This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients. 499 samples were tested at a smear microscopy center and 200 at a central-level mycobacteriology laboratory. Using mycobacterial cultures as reference, TB-LAMP results were compared with those of LED fluorescent microscopy (LED-FM) and Xpert (R) MTB/RIF. At the microscopy center, TB-LAMP sensitivity was higher than that of LED-FM (81.5% [95% CI, 74.5-87.6] versus 69.4% [95% CI, 62.2-76.6]), but lower than that of the Xpert assay (95.5% [95% CI 92.3-98.8]). At the central-level laboratory, TB-LAMP sensitivity (92.8% [95% CI, 87.6-97.9]) was comparable to that of Xpert (90.7% [95% CI, 85.0-96.5]) using stored sample. No significant difference in terms of specificity between TB-LAMP and Xpert assays was observed in both study sites. In conclusion, our data demonstrate that TB-LAMP could be implemented at microscopy centers in Cambodia to detect TB patients. In addition, TB-LAMP can be a better choice to replace smear microscopy for rapid TB diagnosis of new presumptive TB patients, in settings with relative low prevalence of drug-resistant TB and difficulties to implement Xpert assay. |
| نوع الوثيقة: |
text |
| اللغة: |
English |
| Relation: |
https://www.documentation.ird.fr/hor/fdi:010079122; oai:ird.fr:fdi:010079122; Cheng S., Pheng S. H., Seiha H., Marks G. B., Banuls Anne-Laure, Mao T. E., Kerleguer A. Evaluation of loopamp assay for the diagnosis of pulmonary tuberculosis in Cambodia. 2020, 2020, 6828043 [7 p.] |
| الاتاحة: |
https://www.documentation.ird.fr/hor/fdi:010079122 |
| رقم الانضمام: |
edsbas.C9DCF990 |
| قاعدة البيانات: |
BASE |